MESO "Specialized" Testing Group Fund

[Spaceman Spiff]

Bioactivity per mg of growth hormone in a sample sounds like a good test.

Its good. I can't imagine this being a regular test but let's do it for fun. I can probably send a vial of serostim for baseline and then another

Honestly, I'm losing my confidence in @Ghoul. I gave him the benefit of the doubt but I think the opinions on him might be true after all. Disappointing to be honest. But I know that I'm naive as hell so I'm not really surprised of my judgement error.

@Ghoul proved me and many other users right. Thanks for the ego stroke.

He pulled this NMR shit as a form of evidence that we are incorrect. He says he knows where people test it but can't even give ideas on where to get it done.

He doesn't belong here. Just talks the talk.

He could have been right this whole time. But he won't pop his head in here until the test results are posted(and if they are in his favor).

He has spent enough time on this forum, being tagged by multiple users. He is avoiding all of us . As I have said. I. Suspect he lives his "real life" like this. Although, it appears this is his real life.

Now we will have this thread to forever reference that ghoul shouldn't be giving advice/input on shit over his head.

 

[readalot]

YOU'RE the group. Decide for us.

I'll see if Jano responds. Regardless, I will start working out how to engage with a contract lab, what their constraints are, and if they need a legit entity with documentation to order. Also will determine the cost per assay (Nb2-11 cell assay for GH bioactivity determination).

ATM, I've got "premium" UG GH billed from a Chinese GMP as well as standard "generic" UG GH I can contribute in addition to my monetary pledge.

 

[BamaCrazy]

Its good. I can't imagine this being a regular test but let's do it for fun. I can probably send a vial of serostim for baseline and then another


@Ghoul proved me and many other users right.

He pulled this NMR shit as a form of evidence that we are incorrect. He says he knows where people test it but can't even give ideas on where to get it done.

He doesn't belong here. Just talks the talk.

He could have been right this whole time. But he won't pop his head in here until the test results are posted(and if they are in his favor).

He has spent enough time on this forum, being tagged by multiple users. He is avoiding all of us . As I have said. I. Suspect he lives his "real life" like this. Although, it appears this is his real life.

Now we will have this thread to forever reference that ghoul shouldn't be giving advice/input on shit over his head

He is just waiting for the results so he can feed them into an AI tool and ask it to find flaws. Post it here. And then try to recoup his complete loss of credibility by saying I told you so.

 

[readalot]

find flaws

The only potential flaw with the Nb2 cell line assay was covered in the RGA paper I posted. However, it's still accepted as a valid in-vitro assay for GH bioactivity as I understand it. PLEASE someone correct me if that is wrong.

Bioactivity of rhGH is mediated through the prolactin receptor rather than GHR

Does not completely represent the mechanism of action of rhGH, low specificity and time-consuming; Nb2-11 cells are dependent on imports–

 

[Spaceman Spiff]

He is just waiting for the results so he can feed them into an AI tool and ask it to find flaws. Post it here. And then try to recoup his complete loss of credibility by saying I told you so.

It's possible he is correct. If he was, then we can like "well shit, this mother fucker is right".


But he has a fear of being wrong. He is that person that does not grow from learning from his own mistakes/failures.

I know I'm argumentative/debating online but this type of action speeds up information being thrown around and increases learning/understanding.

I used to spend a lot of time on bodybuilding.com on this reviewing nutrition, lifestyle changes, etc.


I love this stuff because it facilitates learning if you are right or not.



On a serious note, I'll throw in 300 dollars for testing.

Don't worry @Ghoul. I don't need your donation to contribute more to harm reduction.

 

[readalot]

It's possible he is correct. If he was, then we can like "well shit, this mother fucker is right".

Correct about what? The structure of UG vs Pharma?

As I understand it, we are moving forward with bioactivity test. If the UG samples comes back poorly, we won't know why. That is why I was pitching CD along with the bioactivity. However, I agree with the feedback to just do the bioactivity assay. If it comes back good we don't need the CD/structure analysis, although that would be funny if the structure came back different between UG and Pharma yet the bioactivity between them was equivalent statistically. TBD down the road maybe.

 

[bananafeet]

I'll pledge $100 USD. Interested to see the results.

 

[Spaceman Spiff]

.

Correct about what? The structure of UG vs Pharma?

As I understand it, we are moving forward with bioactivity test. If the UG samples comes back poorly, we won't know why. That is why I was pitching CD along with the bioactivity. However, I agree with the feedback to just do the bioactivity assay. If it comes back good we don't need the CD/structure analysis, although that would be funny if the structure came back different between UG and Pharma yet the bioactivity between them was equivalent statistically. TBD down the road maybe.

Structure of hgh. Is there even a point ?

Is it even necessary to see bioactivity if we can still measure igf1? This may be a crude way to see if the body does respond to it and increase production

This is your show man, but you may find this useful. In discussion with the guys that handled the Tirz NMR analysis, they offered CD as an option for rHGH that would capture defects they thought likely in UGL rHGH, but be cost effective enough for routine analysis. (unlike NMR)

So he has a connect for a guy who can do NMR testing? Ghoul should be able to connect someone but again is still silent.

 

[janoshik]

Anyone worked with this lab in Germany?

Yourbioanalytical services powerhouse | Development Validation and Routine testing of biologics and drug products

We are a contract service organization specialized in the development and validation of bioassays for pharmaceutical potency testing. Our services are performed under GMP and GLP certification to meet regulatory guidance for pre-market and on-market products.

bioassay-online.com

https://www.pharmaceutical-networking.com/wp-content/uploads/2023/10/Cell-based-Potency-Assays-HGH.pdf

@janoshik
Any recommendation if they need a legit entity to order the Nb2 cell line assay?

No idea what your question is, apologies. Please, clarify.

 

[readalot]

Is it even necessary to see bioactivity if we can still measure igf1? This may be a crude way to see if the body does respond to it and increase production

I get your point. That's where the serum GH test 3 hours after 10 IU IM injection is useful as agreed upon blood test to do.

In vitro bioassay would just be another confirmation. I have no idea if UG GH can pop as a false positive on the serum GH sandwich immunoassay.

 

[readalot]

No idea what your question is, apologies. Please, clarify.

If a contract lab needs a legal entity with proper company paperwork in order to purchase the Nb2-11 cell line bioassay, do you have any recommendations? For example, could we work through your company to order the tests if the CRO wants a real customer with legal paperwork? Could pay you a contracting fee?

 

[readalot]

There is a group called project clear gear, with this exact premise in mind. Would everyone be open to operate within that group? Already have a discord setup if everyone is willing.

Whatever the team wants. I won't be on discord. Too much as it is.

 

[Wrangel7]

Best wishes to the group. If I have enough to spare, may contribute at some point.

 

[BigDadd7]

Whatever the team wants. I won't be on discord. Too much as it is.

My man... you ARE the team. Choose the test.

 

[readalot]

My man... you ARE the team. Choose the test.

I made a proposal and ID'ed the test.

I am receptive to feedback from the last go around where I was told I wasn't building the community / TEAM.

 

[BigDadd7]

I made a proposal and ID'ed the test.

I am receptive to feedback from the last go around where I was told I wasn't building the community / TEAM.

I appreciate your effort in spearheading this!

If we're taking votes, you have my vote.

 

[janoshik]

If a contract lab needs a legal entity with proper company paperwork in order to purchase the Nb2-11 cell line bioassay, do you have any recommendations? For example, could we work through your company to order the tests if the CRO wants a real customer with legal paperwork? Could pay you a contracting fee?

I don't think we can have non-employees handle samples sent on our behalf, unfortunately. Thin ice legally

 

[readalot]

I don't think we can have non-employees handle samples sent on our behalf, unfortunately. Thin ice legally

Send all samples to you for consolidation then one ship from you to CRO?

 

[AlexDavis43]

He is just waiting for the results so he can feed them into an AI tool and ask it to find flaws. Post it here. And then try to recoup his complete loss of credibility by saying I told you so.

"ChatGPT phrase the answer like a layman would say it"

No obvious AI copy & paste walls of text anymore

 

[AlexDavis43]

In vitro bioassay would just be another confirmation. I have no idea if UG GH can pop as a false positive on the serum GH sandwich immunoassay.

Antibodies typically undergo a validation process to prevent this

If a contract lab needs a legal entity with proper company paperwork in order to purchase the Nb2-11 cell line bioassay, do you have any recommendations? For example, could we work through your company to order the tests if the CRO wants a real customer with legal paperwork? Could pay you a contracting fee?

Also check out the BaF/GM assay like in this paper

Seems like it's been around longer and may be more common