Lobster HGH no vacuum - fridge vs no fridge test

[Spaceman Spiff]

It's all from the same batch.

My bad

 

[Spaceman Spiff]

I guess there Turkish people know about making hgh.

There is more to the country than cosmetic surgeries, kebabs, and rugs

 

[dinfar1337]

Room temp and no vacuum does seem more degraded no? Even room temp with vacuum seems fine.

yes

seems like fridge vs no fridge has become more the experiment than vacuum vs no vacuum when you look at it overall. but no fridge has just made the degradation faster and shown the difference.

what it shows; you probably shouldnt care too much under 5 years with no fridge and shouldn't care for it under 20+ years as long as you have +95% hgh + fridge with that stastical difference. thats atleast how im reading it

 

[AlexDavis43]

yes

seems like fridge vs no fridge has become more the experiment than vacuum vs no vacuum when you look at it overall. but no fridge has just made the degradation faster and shown the difference.

what it shows; you probably shouldnt care too much under 5 years with no fridge and shouldn't care for it under 20+ years as long as you have +95% hgh + fridge with that stastical difference. thats atleast how im reading it

I'm reading it as: they're all the same

Less than one percentage point difference is well within the margin of error

C & D are replicates and the difference between C & D is bigger than any other comparison in these data

 

[dinfar1337]

I'm reading it as: they're all the same

Less than one percentage point difference is well within the margin of error

C & D are replicates and the difference between C & D is bigger than any other comparison in these data

i hope my lines can make it more obvious. 3.64 is the only irregular and is to be ignored.

everything was pretty much the same besides no vacuum no fridge

 

[AlexDavis43]

i hope my lines can make it more obvious. 3.64 is the only irregular and is to be ignored.

everything was pretty much the same besides no vacuum no fridge

View attachment 354652

The difference between fridge + vacuum and no fridge + no vacuum is 0.92

The difference between C and D is 0.96

In a lab setting this would put 0.96 as the baseline (like when you press tare on a scale); anything smaller than 0.96 is effectively zero

 

[readalot]

i hope my lines can make it more obvious. 3.64 is the only irregular and is to be ignored.

everything was pretty much the same besides no vacuum no fridge

View attachment 354652

Purity:
No sig difference between groups.

Dimer+:
One or more groups are sig different but not in the trend you would expect.

Seems more like an analysis of Jano's method precision with this particular measurement. Makes no sense for other groups to have lower "dimer+" than control.

Caveat: n=2 for each group. No obvious outliers to throw out.

 

[dinfar1337]

Jano's method precision with this measurement.

seems like detection issues but no worries in those amounts.

thanks for your analysis.

i was just comparing higher mg amount >= lower purity vs lower mg amount >= higher purity but we end up getting pretty much the same. so i was thinking lobster manufacturing proccess at c and d making it a irregurlar and adding a dick to it

 

[readalot]

seems like detection issues but no worries in those amounts

Yep, just stating the obvious statistical results. Significance vs practical importance.

 

[dinfar1337]

Yep, just stating the obvious statistical results. Significance vs practical importance.

maybe you can make same model for

baseline >=3.41mg

no vacuum no fridge =<3.31mg

purity fridge & no fridge + vacuum = avg +- variance (-) no vacuum no fridge = avg +- variance

avg +- variance in = baseline mg - no vacuum no fridge (+) purity baseline - purity no vacuum no fridge.

and * with 10 or 20 to get 5 or 10 years ahead from 6 months difference.

 

[readalot]

seems like detection issues but no worries in those amounts.

thanks for your analysis.

i was just comparing higher mg amount >= lower purity vs lower mg amount >= higher purity but we end up getting pretty much the same. so i was thinking lobster manufacturing proccess at c and d making it a irregurlar and adding a dick to it

Good point on the mg amount for sample C. That might qualify for outlier Q test but too lazy to do it. Good eye and very perceptive. I just ran the purity and dimer+ columns.

 

[readalot]

maybe you can make same model for

baseline >=3.41mg

no vacuum no fridge =<3.31mg

purity fridge & no fridge + vacuum = avg +- variance (-) no vacuum no fridge = avg +- variance

avg +- variance in = baseline mg - no vacuum no fridge (+) purity baseline - purity no vacuum no fridge.

and * with 10 or 20 to get 5 or 10 years ahead from 6 months difference.

I'm good.

 

[dinfar1337]

I'm good.

its just simple catch.

the purity scaling lower in % + less mg content im uncertain if will be non linear or linear.

im also too lazy so i will drop it haha.

at the top of my head it will be around 1iu drop every 3 years no vacuum no fridge.

 

[readalot]

its just simple catch.

the purity scaling lower in % + less mg content im uncertain if will be non linear or linear.

im also too lazy so i will drop it haha.

at the top of my head it will be around 1iu drop every 3 years no vacuum no fridge.

We are just talking to each other. Nice chatting.

The dimer+ results have me scratching my head so not much point (in my mind) in throwing more work at it with such lower rep numbers.

 

[readalot]

at the top of my head it will be around 1iu drop every 3 years no vacuum no fridge

Good idea and would be fun to superpose trend over literature data.

 

[dinfar1337]

Good idea and would be fun to superpose trend over literature data.

it seems like i will pass the ball onto @Ghoul

he can do that and strengthen his position on no vaccum difference.

probably have to find stronger differantion methods than the one i freestyled

 

[TallandSmall]

A&B being the only one with much dimmer is a real head scratcher for us bubbles boys. Feels like a crap shoot trying to get it right. Always nice to find out that it probably doesn’t matter in any kind of practical way. Do I take my Hgh out of the fridge to make room for more food?

 

[Spaceman Spiff]

I like how the dipshit is so silent about these test results. He hasn't said anything, just like his NMR testing.

He brought 2 arguments on in this forum but he has gone silent when things are underway.

He should be looked as someone that isn't a credible source of information and newbies should learn

 

[AlexDavis43]

it seems like i will pass the ball onto @Ghoul

he can do that and strengthen his position on no vaccum difference.

probably have to find stronger differantion methods than the one i freestyled

hahaha oh shit

"These data suggest best practice for maximum purity and minimum dimer of your GH, is to release the vacuum and store in the fridge"

 

[Spaceman Spiff]

One problem is that unlike the studies attached, UGL vials don't have identical starting amounts of rHGH. Filtering prior to analysis removes large aggregates. So if you know with certainty there was precisely 1mg of rHGH in each vial, you can account for this loss and add the "disappeared" rHGH to the measured amount of degradation. But with UGL if one vial is 1.25mg and the other is 1.12mg there's really
no way to determine how much is lost to large aggregates that formed immediately after reconstitution and caught in the filter.

There's also no vacuum sealed control vial to compare these to, unless Jano has one lying around he can use.

Still though, it should be possible to measure greater degradation in the unrefrigerated vial vs the refrigerated one over a week's time.

If you dig into the rHGH oxidation / stability studies, what oxidation really does is make rHGH unfold at much lower temperatures than if it's not oxidized. So the room temp stored vial should degrade faster than the refridgerated one.

Since you are so knowledgeable via chat gpt are you going to make any comments on these results? Or are you going to talk shit about @janoshik and retreat back to NMR structure evaluation which you failed to assist with?

Anything that requires more thinking than a search engine you seem to go silent.



I look forward to your response