Is anyone filtering with a 25mm 0.22um filter?

[iloveblondes666]

I've read a lot especially @Ghoul work and I'm sold on filtering the GLP-1 now; Matter of fact I think it's even more important than HGH,

whereas with GH there may be some room for debate for the effectiveness/necessity of the filtering, with GLP-1s I think it's a no brainer and the easiest and best thing you could possibly do to make sure you can use these compounds for a long time without worrying about immunogenicity.

One single filtering reduces 95% of aggregates and brings UGL/compound pharmacy quality to almost pharma standards? Sign me in.

Now to my question, I already know 4mm or 13mm are the best sized filters with the minimum liquid uphold loss, but I can't for the life of me find a good source in europe or one from the USA which is not absurdly priced (cobetter doesn't ship to my country surprisingly)

I've found some good priced 0.22 PES 25mm ones made by ISOLAB at around 45$ for 50 pieces,

but the problem I hear with 25mm is you're gonna have a lot of water upholding, some say up to 0.5ml.

I'm planning to use these on retatrutide 30mg, but I ll also buy lower dosage retatrutide like 10mg if it matters because I'm keen on filtering.

Will also probably try filtering my GH why not but that's as a separate less important note.

I also have unlimited bac water so I'll fill every vial with 3ml plus, but I wanna hear your experiences with using 25mm filters. I also couldn't find how reliable air / water purging is if the liquid gets stuck, thanks!

 

[Photon]

I've read a lot especially @Ghoul work and I'm sold on filtering the GLP-1 now; Matter of fact I think it's even more important than HGH,

whereas with GH there may be some room for debate for the effectiveness/necessity of the filtering, with GLP-1s I think it's a no brainer and the easiest and best thing you could possibly do to make sure you can use these compounds for a long time without worrying about immunogenicity.

One single filtering reduces 95% of aggregates and brings UGL/compound pharmacy quality to almost pharma standards? Sign me in.

Now to my question, I already know 4mm or 13mm are the best sized filters with the minimum liquid uphold loss, but I can't for the life of me find a good source in europe or one from the USA which is not absurdly priced (cobetter doesn't ship to my country surprisingly)

I've found some good priced 0.22 PES 25mm ones made by ISOLAB at around 45$ for 50 pieces,

but the problem I hear with 25mm is you're gonna have a lot of water upholding, some say up to 0.5ml.

I'm planning to use these on retatrutide 30mg, but I ll also buy lower dosage retatrutide like 10mg if it matters because I'm keen on filtering.

Will also probably try filtering my GH why not but that's as a separate less important note.

I also have unlimited bac water so I'll fill every vial with 3ml plus, but I wanna hear your experiences with using 25mm filters. I also couldn't find how reliable air / water purging is if the liquid gets stuck, thanks!

Flush the filter with bac
Something is wrong if your bac gets stuck

 

[HUGE McBIGLARGE]

I flush the filter with BAC for three drops (I guess that cleans it?).

Once I’ve passed through all of the peptide, I pass through plain BAC to flush more of the peptide through. Then I flush air through to try to get more BAC out of the filter.

At the moment I reconstitute all peptides with around 4ml of BAC. More is better.

As for filter size, it doesn’t really matter if you flush BAC and air through after.

 

[Billbo98]

Nah I don’t filter really don’t see a reason why too honestly. Oils sure but peptides and GH no. Labs have all been good so far without doing it.

They actually asked this on Blood Sweat And Gear podcast and Scott basically was like uhhh…no lol

 

[HUGE McBIGLARGE]

Nah I don’t filter really don’t see a reason why too honestly. Oils sure but peptides and GH no. Labs have all been good so far without doing it.

They actually asked this on Blood Sweat And Gear podcast and Scott basically was like uhhh…no lol

Yeah. Filtering is almost certainly pointless. But it could prevent some pretty bad outcomes, and it only adds maybe two minutes of prep per reconstitution, and it costs about a dollar per filter.

So filtering is low cost, with a high chance of doing fuck all, but a low chance of filtering toxins and aggregates.

 

[Billbo98]

Yeah. Filtering is almost certainly pointless. But it could prevent some pretty bad outcomes, and it only adds maybe two minutes of prep per reconstitution, and it costs about a dollar per filter.

So filtering is low cost, with a high chance of doing fuck all, but a low chance of filtering toxins and aggregates.

I mean for those truly concerned with their sources purity and what not I understand it and those who just want the extra protection more power to them but I’ve never known of people filtering until I’ve seen all the posts here. Maybe I’m missing something here but yeah just my opinions

 

[RoxasShadow]

To me it takes zero time and it's worth even if it's just for the mental benefit (and we know it is not just that, based on @Ghoul's sharing). The only side I see is the little bit of drug that remains in the syringe, which for rhGH is quite a waste (PES, 0.22μm, 13mm + 1ml Reduced Dead Space Syringe Barrel + BD 25G 1 inch).

 

[ParquetPatquet]

Filtering is a cheap way to remove harmful bacteria and fungus from your solution, if they are present. Same logic behind wiping the top of your vial before injecting. And It's less about trusting your source (unless your source is Lilly) , but more about risk reduction.

 

[RoxasShadow]

To me it takes zero time and it's worth even if it's just for the mental benefit (and we know it is not just that, based on @Ghoul's sharing). The only side I see is the little bit of drug that remains in the syringe, which for rhGH is quite a waste (PES, 0.22μm, 13mm + 1ml Reduced Dead Space Syringe Barrel + BD 25G 1 inch).

Injected 2.5ml bac water in an hgh vial and between a swap of needles (draw and inject bac water with a 21g, swap to a clean 18g to draw the solution and inject with a 25g into a 3ml pen cartridge), and the filter (see pic below) actually ended up with about with about 190ml in the final cartridge.

Any tips from @Ghoul or someone?

 

[Billbo98]

Y

Injected 2.5ml bac water in an hgh vial and between a swap of needles (draw and inject bac water with a 21g, swap to a clean 18g to draw the solution and inject with a 25g into a 3ml pen cartridge), and the filter (see pic below) actually ended up with about with about 190ml in the final cartridge.

Any tips from @Ghoul or someone?

View attachment 377996

yeah this filtering is a giant waste of time and product for peptides lmao

 

[ParquetPatquet]

Injected 2.5ml bac water in an hgh vial and between a swap of needles (draw and inject bac water with a 21g, swap to a clean 18g to draw the solution and inject with a 25g into a 3ml pen cartridge), and the filter (see pic below) actually ended up with about with about 190ml in the final cartridge.

Any tips from @Ghoul or someone?

View attachment 377996

Flush what's remaining with air : detach filter, draw air up to 3ml, attach filter, flush the rest of the liquid on the cartridge.

 

[justanoob]

If one wanted to learn how to filter, could someone explain step by step or is there a video tutorial of sorts? Also, roughly how long does it take per vial? Thanks

 

[Billbo98]

If one wanted to learn how to filter, could someone explain step by step or is there a video tutorial of sorts? Also, roughly how long does it take per vial? Thanks

Buy filters, make sure sterile. 22um PTFE 13 or 25mm. Suck liquid up through pin without filter, add filter then push into sterile vial.

 

[Beginnerweak]

I've read a lot especially @Ghoul work and I'm sold on filtering the GLP-1 now; Matter of fact I think it's even more important than HGH,

whereas with GH there may be some room for debate for the effectiveness/necessity of the filtering, with GLP-1s I think it's a no brainer and the easiest and best thing you could possibly do to make sure you can use these compounds for a long time without worrying about immunogenicity.

One single filtering reduces 95% of aggregates and brings UGL/compound pharmacy quality to almost pharma standards? Sign me in.

Now to my question, I already know 4mm or 13mm are the best sized filters with the minimum liquid uphold loss, but I can't for the life of me find a good source in europe or one from the USA which is not absurdly priced (cobetter doesn't ship to my country surprisingly)

I've found some good priced 0.22 PES 25mm ones made by ISOLAB at around 45$ for 50 pieces,

but the problem I hear with 25mm is you're gonna have a lot of water upholding, some say up to 0.5ml.

I'm planning to use these on retatrutide 30mg, but I ll also buy lower dosage retatrutide like 10mg if it matters because I'm keen on filtering.

Will also probably try filtering my GH why not but that's as a separate less important note.

I also have unlimited bac water so I'll fill every vial with 3ml plus, but I wanna hear your experiences with using 25mm filters. I also couldn't find how reliable air / water purging is if the liquid gets stuck, thanks!

Why is it more important to filter GLP1 rather than HG, I thought HG was the important one to filter since it's injected everyday and the body can start rejecting it or something like that due to the aggregates.

Edit : believe I'm right in saying that HGH is more important to filter because GLP-1 agonists show lower immunogenicity than HGH, with antibodies rarely impacting efficacy.

"

• HGH Immunogenicity: Recombinant HGH often induces ADAs in 7-22% of patients, depending on the formulation. Aggregates in HGH products significantly enhance immunogenicity, as shown in mouse models where stressed (e.g., agitated or freeze-thawed) formulations produced stronger immune responses. In some cases, ADAs neutralize HGH activity, reducing therapeutic efficacy. Pituitary-derived or early recombinant forms had even higher rates (up to 81% in monkey studies), though modern versions are improved.
• GLP-1 Immunogenicity: GLP-1 receptor agonists show lower overall immunogenicity. For example:
  • Exenatide (low homology to human GLP-1): 38-60% ADA incidence, but mostly low-titer and non-neutralizing, with rare impact on efficacy (3-6% of cases).
  • Liraglutide (high homology): 9-13% ADA rate, with no significant efficacy loss.
  • Newer ones like tirzepatide: Neutralizing ADAs in only 1.9-2.1% of patients.GLP-1 analogs are often engineered (e.g., with fatty acid chains or fusions) to minimize immune recognition, and aggregates are less frequently cited as a driver compared to HGH."

 

[SubparMarioBro]

Why is it more important to filter GLP1 rather than HG, I thought HG was the important one to filter since it's injected everyday and the body can start rejecting it or something like that due to the aggregates.

Edit : believe I'm right in saying that HGH is more important to filter because GLP-1 agonists show lower immunogenicity than HGH, with antibodies rarely impacting efficacy.

"

• HGH Immunogenicity: Recombinant HGH often induces ADAs in 7-22% of patients, depending on the formulation. Aggregates in HGH products significantly enhance immunogenicity, as shown in mouse models where stressed (e.g., agitated or freeze-thawed) formulations produced stronger immune responses. In some cases, ADAs neutralize HGH activity, reducing therapeutic efficacy. Pituitary-derived or early recombinant forms had even higher rates (up to 81% in monkey studies), though modern versions are improved.
• GLP-1 Immunogenicity: GLP-1 receptor agonists show lower overall immunogenicity. For example:
  • Exenatide (low homology to human GLP-1): 38-60% ADA incidence, but mostly low-titer and non-neutralizing, with rare impact on efficacy (3-6% of cases).
  • Liraglutide (high homology): 9-13% ADA rate, with no significant efficacy loss.
  • Newer ones like tirzepatide: Neutralizing ADAs in only 1.9-2.1% of patients.GLP-1 analogs are often engineered (e.g., with fatty acid chains or fusions) to minimize immune recognition, and aggregates are less frequently cited as a driver compared to HGH."

You’re comparing two different things here. For hGH you’re looking at total prevalence of anti-drug antibodies whereas for GLP-1s you’re focusing on neutralizing antibodies. Kind of like comparing a bag of wheat and a loaf of bread.

The only cases that ultimately matter for either are antibodies with actual clinical effect.