Injectable Korean Glutathione, been doing it wrong?

[aromatizer]

I ordered some Korean Glutathione, which arrived in like 2 days. Searched the threads for info on dosage etc. Also got Gemini AI to translate the instructions from Korean.

It seems that once reconstituted the instructions are clear that it needs to be used straight away and any unused portion to be discarded. Gemini agreed, explaining that once reconstituted glutathione starts to degrade very quickly. No amount of BAC water will prevent the degradation and that using degraded product is bad. Unless the water has been specifically de-oxidised, the oxygen disolved in the water will degrade the glutathione. Secondly the vial wont remain under vacuum as it is repeatedly used.

That's a bummer, I have 1200mg vials and was planning on only using 2-300mg at a time to start. thoughts?

Here is a snippet of the conversation with the AI:

------

1. The "Vacuum" Myth​

You are correct that the vacuum keeps air out. However, when you inject water (even Bac Water) into the vial, the water itself contains dissolved oxygen.

• Unless you are using specialized laboratory-grade "degassed" water, the oxygen dissolved in that liquid is enough to start destroying the Glutathione immediately.
• The moment the powder dissolves, it begins turning into GSSG (Oxidized Glutathione).

2. The Problem with "A Few Days"​

Glutathione is incredibly unstable once mixed.

• Within hours: A significant portion of the active "Reduced Glutathione" (GSH) oxidizes into GSSG.
• After 24+ hours: Even in the fridge and in the dark, the solution degrades further.
• The Result: You are no longer injecting a potent antioxidant. You are injecting Oxidized Glutathione, which is the "waste product" your body creates after fighting free radicals. Injecting high levels of oxidized glutathione can actually increase oxidative stress in your body, which is the exact opposite of what you want.
  
  

 

[xrayphoton13]

In AI we trust.

 

[bananafeet]

They are packed with argon not a vacuum which is superior.

Mine were buffered with bicarb which extends the shelf life when refrigerated to two weeks with BA water.

Make sure you make the concentration 200-300mg/ml. Any more and it burns like hell and you may even lose some skin. I've seen bad shots on here from high concentrations of glutathione.

You can take 600-1200mg per week no issues. I'd just take a few vials and have a break. No need to be on it continuously. The deep IM injections are so uncomfortable you won't want to do it much anyway....

 

[Photon]

I ordered some Korean Glutathione, which arrived in like 2 days. Searched the threads for info on dosage etc. Also got Gemini AI to translate the instructions from Korean.

It seems that once reconstituted the instructions are clear that it needs to be used straight away and any unused portion to be discarded. Gemini agreed, explaining that once reconstituted glutathione starts to degrade very quickly. No amount of BAC water will prevent the degradation and that using degraded product is bad. Unless the water has been specifically de-oxidised, the oxygen disolved in the water will degrade the glutathione. Secondly the vial wont remain under vacuum as it is repeatedly used.

That's a bummer, I have 1200mg vials and was planning on only using 2-300mg at a time to start. thoughts?

Here is a snippet of the conversation with the AI:

------

1. The "Vacuum" Myth​

You are correct that the vacuum keeps air out. However, when you inject water (even Bac Water) into the vial, the water itself contains dissolved oxygen.

• Unless you are using specialized laboratory-grade "degassed" water, the oxygen dissolved in that liquid is enough to start destroying the Glutathione immediately.
• The moment the powder dissolves, it begins turning into GSSG (Oxidized Glutathione).

2. The Problem with "A Few Days"​

Glutathione is incredibly unstable once mixed.

• Within hours: A significant portion of the active "Reduced Glutathione" (GSH) oxidizes into GSSG.
• After 24+ hours: Even in the fridge and in the dark, the solution degrades further.
• The Result: You are no longer injecting a potent antioxidant. You are injecting Oxidized Glutathione, which is the "waste product" your body creates after fighting free radicals. Injecting high levels of oxidized glutathione can actually increase oxidative stress in your body, which is the exact opposite of what you want.

KR Pharma recons at ~pH 6.4 when used with pharma Hospira bac water, which remains stable in the fridge for months.

US6835811B1 - Extended storage of reduced glutathione solutions - Google Patents

A method of storing solutions of reduced glutathione for extended periods of time, by dissolving reduced glutathione in an aqueous medium having a pH of between 5.0 and 8.0 to produce a reduced glutathione solution; reducing the temperature of the reduced glutathione solution to a predetermined...

patents.google.com

 

[aromatizer]

Gemini still thinks it's not going to work:
----
Here is the breakdown of why the patent works in a lab but fails in a home setting:

1. The Patent's "Secret Ingredient" is NO Oxygen​

If you look closely at the "Detailed Description" of the patent you linked (US6835811B1), it lists a specific requirement for that extended shelf life:

"It is preferred that the solution be stored under a non-oxidizing atmosphere which is free of molecular oxygen (i.e., nitrogen, argon, or a vacuum), and that the solution be prepared using distilled water which is free of metallic ions."

Why this matters to you:

• In the Lab (Patent conditions): They use special water that has had all the oxygen removed ("degassed"). They fill the vial with nitrogen gas so no air touches the liquid.
• At Home: When you inject Bacteriostatic Water into the vial, that water is full of dissolved oxygen. The air inside your syringe also contains oxygen.
• The Result: Even though your vial is buffered (pH 5.0–8.0) and you put it in the fridge (<15°C), the oxygen you introduced during mixing will immediately start attacking the glutathione.

2. Buffering (Sodium Bicarbonate)​

Your vial is buffered. The label lists Sodium Bicarbonate (baking soda) as an ingredient.

• The Good News: This keeps the pH in the stable range (around 6.0–7.0) mentioned in the patent, preventing it from being too acidic.
• The Bad News: Buffering helps prevent acid-hydrolysis (breakdown from acid), but it does not stop oxidation (breakdown from air).

 

[Photon]

Gemini still thinks it's not going to work:
----
Here is the breakdown of why the patent works in a lab but fails in a home setting:

1. The Patent's "Secret Ingredient" is NO Oxygen​

If you look closely at the "Detailed Description" of the patent you linked (US6835811B1), it lists a specific requirement for that extended shelf life:


Why this matters to you:

• In the Lab (Patent conditions): They use special water that has had all the oxygen removed ("degassed"). They fill the vial with nitrogen gas so no air touches the liquid.
• At Home: When you inject Bacteriostatic Water into the vial, that water is full of dissolved oxygen. The air inside your syringe also contains oxygen.
• The Result: Even though your vial is buffered (pH 5.0–8.0) and you put it in the fridge (<15°C), the oxygen you introduced during mixing will immediately start attacking the glutathione.

2. Buffering (Sodium Bicarbonate)​

Your vial is buffered. The label lists Sodium Bicarbonate (baking soda) as an ingredient.

• The Good News: This keeps the pH in the stable range (around 6.0–7.0) mentioned in the patent, preventing it from being too acidic.
• The Bad News: Buffering helps prevent acid-hydrolysis (breakdown from acid), but it does not stop oxidation (breakdown from air).

Cheapest way is to test with your bloodwork.

I know some people here draw it in a syringe and freeze it, something you can also consider.

 

[hooligansluck]

I've been brewing my own, I make a 30ml vials and have seen good results with no degradation supported with blood work numbers. When I blast it, I run 500mg a day, run it with activated charcoal and my sauna routine. I drink, I'm older and seen good result in liver and kidney numbers.

 

[aromatizer]

I got Grok to dig through forums etc:

"On peptide forums (Reddit r/Peptides, Peptide Discord, LongeCity, etc.), people who notice their GSH turning yellow in 1–2 weeks with bac water often switch to saline + nitrogen/argon blanketing and report clear solution for 4–8+ weeks."

"Benzyl alcohol can accelerate oxidation over weeks"

"No, degraded/oxidized glutathione (mostly GSSG) does not become "counterproductive" or harmful in the sense of being pro-oxidant or toxic — even at very high oxidation levels (80–100% GSSG). It simply becomes ineffective as an antioxidant supplement."

Starts to change colour somewhere over 20% degradation. By 50% has a pale yellow tint.

Should throw out if cloudiness/particles appear. (risk of further breakdown products, not GSSG itself)

 

[Photon]

I got Grok to dig through forums etc:

"On peptide forums (Reddit r/Peptides, Peptide Discord, LongeCity, etc.), people who notice their GSH turning yellow in 1–2 weeks with bac water often switch to saline + nitrogen/argon blanketing and report clear solution for 4–8+ weeks."

"No, degraded/oxidized glutathione (mostly GSSG) does not become "counterproductive" or harmful in the sense of being pro-oxidant or toxic — even at very high oxidation levels (80–100% GSSG). It simply becomes ineffective as an antioxidant supplement."

If it turns yellow I'll throw it away.
I have vials that are 5 to 6 months old that are crystal clear.

 

[aromatizer]

More useful tidbits

• Glutathione solubility tops out around 400–500 mg/mL when pH-adjusted properly, but at that concentration it stings a lot and can be thick.
• 200 mg/mL is the sweet spot used by U.S. compounding pharmacies (e.g., Empower, Hallandale) and the vast majority of longevity/peptide users doing daily or EOD subQ shots.

 

[aromatizer]

Cheapest way is to test with your bloodwork.

What changes would you expect to see in the blood work?

 

[gainslol]

What changes would you expect to see in the blood work?

You can test glutathione levels in the blood. It would increase.

 

[Sampei]

Are we really at a this Level of retardiness that we believe anything fucking AI says?

Gluthatione degradation test ran at jano:

Minimal degradation well past 30+ days.

Keel playing with AI

 

[RocketSurge0n]

I've been brewing my own, I make a 30ml vials and have seen good results with no degradation supported with blood work numbers. When I blast it, I run 500mg a day, run it with activated charcoal and my sauna routine. I drink, I'm older and seen good result in liver and kidney numbers.

How long would you say it takes to boost your numbers?