Oily HGH

[ineedhgh878]

I recently received an order of h36 yellow caps from WWB and apon reconstitution, noticed that when injecting hospira BAC into the vial, the drops would sort of slide on the surface of the bac water in the hgh vial before joining with the rest of the water. It’s not visible in the video bc it was quite hard to capture but also it looked oily when injecting water into the vial as seen in the video. Is this normal? It also had tiny bumps and specs all over the vial that went away after throwing it into the fridge for 40 min and then was crystal clear after. Any advice or words would be nice because I’ve searched for this and have no clue what it would be.

 

[ineedhgh878]

Here is the oily injection video since it didn’t attach to the other post.
View attachment IMG_7956.mov

 

[Ghoul]

What was the orientation of the vial when injecting water?

Was it done with easy touch syringe in the video?

 

[Ghoul]

What kind of Bac water are you using

Hospira.

 

[Ateam2023]

Hospira.

I didn't delete it fast enough

 

[ineedhgh878]

What was the orientation of the vial when injecting water?

Was it done with easy touch syringe in the video?

All variables were vial was reconstituted with a 5/8in easy touch insulin syringe at a 30 degree angle and during the first ml of bac the needle was against the side of the glass and gently let trickle down the side of the vial not squirted directly on the lipholized puck. The 2nd and 3rd ml of bac was slowly injected into it at an angle as well. Bac was room temp, brand new, never stored in a fridge and was fresh from a reliable and well known source. The gh was about 2 hours outside of the mail- package so room temp.

 

[Ghoul]

All variables were vial was reconstituted with a 5/8in easy touch insulin syringe at a 30 degree angle and during the first ml of bac the needle was against the side of the glass and gently let trickle down the side of the vial not squirted directly on the lipholized puck. The 2nd and 3rd ml of bac was slowly injected into it at an angle as well. Bac was room temp, brand new, never stored in a fridge and was fresh from a reliable and well known source. The gh was about 2 hours outside of the mail- package so room temp.

One last question. How, *exactly* did you deal with air bubbles after drawing up water from the BAC vial, if you did anything?

 

[ineedhgh878]

One last question. How, *exactly* did you deal with air bubbles after drawing up water from the BAC vial, if you did anything?

I inject a ml of air to displace the ml of bac and then draw it up slowly and then pull it out flick it and visually inspect it for air bubbles and then hold it upright and push out any air if any at the tip of the syringe.

 

[Ghoul]

So I was trying to figure out why the aggregates appeared “globular” in your vial. Also the “wavy” appearance when you swirled it in the video was unusual. I thought they might be silicone contamination droplets, however those are round, and your “specks” had irregular edges like rHGH aggregates, but were translucent instead of the usual white:

When I saw the insulin syringe, and the 3.5ml water it came together.

Insulin syringes are notorious for being heavily lubricated with silicone (SO). Multiple strokes increases the amount expelled significantly. With the amount of BAC, I knew that syringe had to be pumped until all the silicone was scraped off the cylinder walls, into the water and out through the needle:





The issue with silicon is it causes proteins to aggregate:






Only one thing can make it much worse, flicking:







Silicone droplets stick to vial walls, and when rHGH comes onto contact with it, unfolds and forms a “translucent” gel aggregate. Movement knocks the aggregate “jellyfish” loose, and now the spot is available for another rHGH monomer to get stuck, unfold, and the process repeats.

This is how it happens inside a prefilled syringe, but the same process occurs in a vial that’s being swirled, or the impact of just putting it on a hard surface, no flicking needed:







Suggest you switch to silicone free syringes for reconstituting, or any 3ml syringe so multiple strokes won’t be needed, to avoid this problem.

(The cold made them “disappear” because gelataneous aggregates and silicone droplets get denser in cold, sink to the bottom, and are essentially invisible in the solution. )

 

[funsizedtrouble1234]

God damn @Ghoul! Impressive. Are you a scientist who cares about helping the world out or something?

 

[Duck Eggs]

So I was trying to figure out why the aggregates appeared “globular” in your vial. Also the “wavy” appearance when you swirled it in the video was unusual. I thought they might be silicone contamination droplets, however those are round, and your “specks” had irregular edges like rHGH aggregates, but were translucent instead of the usual white:

When I saw the insulin syringe, and the 3.5ml water it came together.

Insulin syringes are notorious for being heavily lubricated with silicone (SO). Multiple strokes increases the amount expelled significantly. With the amount of BAC, I knew that syringe had to be pumped until all the silicone was scraped off the cylinder walls, into the water and out through the needle:

View attachment 369216



The issue with silicon is it causes proteins to aggregate:



View attachment 369222


Only one thing can make it much worse, flicking:



View attachment 369219



Silicone droplets stick to vial walls, and when rHGH comes onto contact with it, unfolds and forms a “translucent” gel aggregate. Movement knocks the aggregate “jellyfish” loose, and now the spot is available for another rHGH monomer to get stuck, unfold, and the process repeats.

This is how it happens inside a prefilled syringe, but the same process occurs in a vial that’s being swirled, or the impact of just putting it on a hard surface, no flicking needed:



View attachment 369223



Suggest you switch to silicone free syringes for reconstituting, or any 3ml syringe so multiple strokes won’t be needed, to avoid this problem.

(The cold made them “disappear” because gelataneous aggregates and silicone droplets get denser in cold, sink to the bottom, and are essentially invisible in the solution. )

Interesting. I just had 2 vials of peak QSC HCG aggregate, but the first vial from the kit didn't. Now that you mention it, maybe I only used a slin pin for the first vial, but a junky 3ml for the second two. Through the kit I'll try different variables like killing the vacuum before adding bac water, different syringes, and see if I can find the factor that messes it up. They're 15kiu vials, so I reconstitute with 3ml, then spread that across 3 insulin carts with an additional ml of bac water in each (5kiu/2ml), and freeze the two carts I'm not using. People get bent out of shape about freezing, but I've never noticed any visible effects or physiological effects from storing reconstituted H G frozen. To be fair, that was always with Eutrig 5k iu vials, but I've been hesitant to return to Indian HCG as I bought that particular Eutrig batch as it was on point while most of the other manufacturers were all over the place. I've always wondered if Chinese HCG is rHCG, vs HP like Indian.

 

[Ghoul]

Interesting. I just had 2 vials of peak QSC HCG aggregate, but the first vial from the kit didn't. Now that you mention it, maybe I only used a slin pin for the first vial, but a junky 3ml for the second two. Through the kit I'll try different variables like killing the vacuum before adding bac water, different syringes, and see if I can find the factor that messes it up. They're 15kiu vials, so I reconstitute with 3ml, then spread that across 3 insulin carts with an additional ml of bac water in each (5kiu/2ml), and freeze the two carts I'm not using. People get bent out of shape about freezing, but I've never noticed any visible effects or physiological effects from storing reconstituted H G frozen. To be fair, that was always with Eutrig 5k iu vials, but I've been hesitant to return to Indian HCG as I bought that particular Eutrig batch as it was on point while most of the other manufacturers were all over the place. I've always wondered if Chinese HCG is rHCG, vs HP like Indian.

Most of the time, assuming proper BAC type and volume is used, visible aggregation is the result of protein degradation from handling of the vial. I think the wild variation in Indian HCG, considering this is seen even in international brands that produce many meds for the US and routinely inspected by the FDA, is down to the extreme heat the product is exposed to in the summer. I think the best practice for Indian brands would be to order in January/March, and request a manufacturing date no older than 4 months.

QSC was recombinant.

 

[Elektrobot]

like killing the vacuum before adding bac water

Make sure to vent the vial if you remove the vacuum so the air can be displaced. Otherwise you're going to make a hgh geyser