Cloudy/Lumpy HGH

[Ghoul]

First tried my standard cobetter 13mm 0.1 with 0.2 prefilter that works really well with tirzepatide and always leaves a crystal clear solution.

I honestly suspected somehow it had been compromised at first because the liquid had particles similar to how the freshly reconstituted SSA 24 iu vial did. I reconstituted with 2.8ml.

I tried to refilter it with a 0.1, thinking that maybe it just got clogged or something. But this didn’t really help much.

So finally I conceeded that maybe the 0.1 really was too much shear force for gh and it was more delicate than I thought, so I moved on to the 0.2 micron filter.

This was better, the solution is clearer but still there are a few floater looking things.

Using 25g 1 inch needle.

I think I can rule out a compromised filter at this stage given the fact I filtered it multiple times.

.1um is too small for rHGH and can cause it to denature (unfold), exposing "sticky" parts that cause aggregates to form.

Tirz is 39 proteins while rHGH is 5x larger, 139 proteins, in a large, complex shape.

Although .1um is ok for Tirz, all pharma protein / peptide drugs are filtered with .22um during manufacturing because that's considered sufficient to remove bacteria without stressing the molecule.

I suggest you get .22um PES filters without a prefilter.

 

[CarvinDC125]

First tried my standard cobetter 13mm 0.1 with 0.2 prefilter that works really well with tirzepatide and always leaves a crystal clear solution.

I honestly suspected somehow it had been compromised at first because the liquid had particles similar to how the freshly reconstituted SSA 24 iu vial did. I reconstituted with 2.8ml.

I tried to refilter it with a 0.1, thinking that maybe it just got clogged or something. But this didn’t really help much.

So finally I conceeded that maybe the 0.1 really was too much shear force for gh and it was more delicate than I thought, so I moved on to the 0.2 micron filter.

This was better, the solution is clearer but still there are a few floater looking things.

Using 25g 1 inch needle.

I think I can rule out a compromised filter at this stage given the fact I filtered it multiple times.

Which batch HGH is this? 10iu, 24iu, etc, and what color is the top?
I had some 15iu black tops that all were slightly cloudy and some had floaters in it. I used them all but two vials. Those two had lots of floaters in it.

 

[Sera]

.1um is too small for rHGH and can cause it to denature (unfold), exposing "sticky" parts that cause aggregates to form.

Tirz is 39 proteins while rHGH is 5x larger, 139 proteins, in a large, complex shape.

Although .1um is ok for Tirz, all pharma protein / peptide drugs are filtered with .22um during manufacturing because that's considered sufficient to remove bacteria without stressing the molecule.

I suggest you get .22um PES filters without a prefilter.

Yes, I do have .22um without a pre filter also. I gave that a try , refiltering it, and same problem.

 

[Ghoul]

Yes, I do have .22um without a pre filter also. I gave that a try , refiltering it, and same problem.

Probably because that solution is already filled with denatured (unfolded) rHGH from the first filtration, and the second filtration promotes aggregation of the already sticky damaged rHGH monomers.

Try filtering a fresh vial with .22um. Also, filter gently without intense pressure.

 

[Sera]

Probably because that solution is already filled with denatured (unfolded) rHGH from the first filtration, and the second filtration promotes aggregation of the already sticky damaged rHGH monomers.

Try filtering a fresh vial with .22um. Also, filter gently without intense pressure.

I just tried a fresh vial, much better. Almost no noticeable particles this time . I think I could really do with 25mm filters for hgh.

Honestly a baffling experience. Even hCG worked well with 0.1!

 

[Spaceman Spiff]

Any evidence that the hgh is indeed folded?

NOPE. No testing


Just constantly spreading this crap with no evidence and refuses to test.

 

[Ghoul]

Any evidence that the hgh is indeed folded?

NOPE. No testing

LOL, so eager to spew your one liner I guess you missed the part where he already ran that rHGH through a .1um filter?

That's 16x the resistance and shear stress of .22um. That's why it's not used by pharma in rHGH production, because unlike .22um, .1um unfolds it.

 

[readalot]

What do you say we all start a special projects MESO fund for testing? Any interest in a separate thread to spread the word?

For any GH "special" testing, I pitch we do it as a group to help build community here at MESO. I will commit $500 (and some product as part of the testing if we do head to head comparisons). Thoughts?

My concern at this point is Jano's bandwidth and people sending in product separately (pharma vs "generic"). Hence, logistically it may make sense for one person to send samples. Anyway, I'll support whatever the team thinks has best chance for credibility and success.

We also probably need trusted members to be trustees for the fund. Perhaps quorum (2 out of 3 trustees) decision to approve funds for a test?

 

[Kaching]

That would be great, as even when i filter the cloudy SSA batch it still stays cloudy.

 

[AlexDavis43]

That would be great, as even when i filter the cloudy SSA batch it still stays cloudy.

Something doesn't sound right

Cloudy? White particulates or lots of small bubbles?

 

[Spaceman Spiff]

What do you say we all start a special projects MESO fund for testing? Any interest in a separate thread to spread the word?

For any GH "special" testing, I pitch we do it as a group to help build community here at MESO. I will commit $500 (and some product as part of the testing if we do head to head comparisons). Thoughts?

My concern at this point is Jano's bandwidth and people sending in product separately (pharma vs "generic"). Hence, logistically it may make sense for one person to send samples. Anyway, I'll support whatever the team thinks has best chance for credibility and success.

We also probably need trusted members to be trustees for the fund. Perhaps quorum (2 out of 3 trustees) decision to approve funds for a test?

Should make an official thread IMO . lets fine some serious people.

 

[verylargeshoes]

What do you say we all start a special projects MESO fund for testing? Any interest in a separate thread to spread the word?

For any GH "special" testing, I pitch we do it as a group to help build community here at MESO. I will commit $500 (and some product as part of the testing if we do head to head comparisons). Thoughts?

My concern at this point is Jano's bandwidth and people sending in product separately (pharma vs "generic"). Hence, logistically it may make sense for one person to send samples. Anyway, I'll support whatever the team thinks has best chance for credibility and success.

We also probably need trusted members to be trustees for the fund. Perhaps quorum (2 out of 3 trustees) decision to approve funds for a test?

I don't fully understand the details of the idea but i'm interested and would be willing to contribute $ and product.

 

[readalot]

I don't fully understand the details of the idea but i'm interested and would be willing to contribute $ and product.

This thread has the details.

R

Post in thread 'The generic vs pharma GH discussion'

May 30, 2025

I'll organize it, will you chip in?

Status?

I was planning on getting some more Serostim so I could run head to head against a "premium" UG GH and a typical UG "generic" GH. The "premium" supposedly tests better against pharma standard with HPLC-MS. I don't have that data and vendor won't share it.

The price Jano quoted me was 400 USD for a pair wise comparison with CD and 600 USD for 3 samples. He stated this was no markup on his part with outside lab. I want to speak with this lab first if we go with CD to confirm near vs far UV and make sure it isn't a mess...

• readalot

• 

Thanks! I started a thread for a community fund so folks can flesh out the details....

R

Thread 'MESO "Specialized" Testing Group Fund'

Jul 10, 2025

** Starting a thread after there was some interest from initial post, and we have been kicking this idea around for a bit. **

What do you say we all start a special projects MESO fund for testing? Idea is to push the envelope beyond standard tests done today to help answer outstanding questions like bioactivity of UG GH.

For any GH "special" testing, I pitch we do it as a group to help build community here at MESO. I will commit $500 (and some product as part of the testing if we do head to head comparisons). Thoughts?

My concern at this point is Jano's bandwidth and people sending in...

• readalot
• #testing anabolic steroids analytical human growth hormone
• Replies: 1,592
• Forum: Analytical Lab Testing

• 

Various additional testing has been kicked around in the context of pharma vs UG GH...

1. NMR / Circular dichroism (HGH structure)
2. Nb2 cell line assay or reporter gene assay (biological activity of GH in IU/mg)
3. Host cell protein assays

 

[Spaceman Spiff]

I don't fully understand the details of the idea but i'm interested and would be willing to contribute $ and product.

This is what you can do to find the background.

Use the search function

Type username @Ghoul
Then type in NMR

He keeps talking about the importance of this test but never talks about doing anything for harm reduction

 

[verylargeshoes]

He keeps talking about the importance of this test but never talks about doing anything for harm reduction

I'm starting to suspect that you two don't get along. ;-)

Just out of curiosity - why should he (or anybody in particular) be obligated to do anything for harm reduction? I don't even know if he uses the stuff we're talking about testing, but it seems like we're all volunteers (or not) here. Contributing information is still useful, if it's mostly correct and relevant.

Thanks for the pointer, I'll get to reading.

 

[Spaceman Spiff]

I'm starting to suspect that you two don't get along. ;-)

Just out of curiosity - why should he (or anybody in particular) be obligated to do anything for harm reduction? I don't even know if he uses the stuff we're talking about testing, but it seems like we're all volunteers (or not) here. Contributing information is still useful, if it's mostly correct and relevant.

Thanks for the pointer, I'll get to reading.

It's not that anyone is obligated.
He constantly uses this in arguments. Folded, stickied, HGH, aggregates, etc. . Can only be confirmed by testing which he claims.

He assumes that people has poor quality but never does any testing to back it up.


When we even ask for a small donation, he goes silent. Accused me of some dumb shit on this forum.

 

[Jron]

I'm running SSA 36 IU GH with some Bac "reconstitution solution" I bought off Amazon. It mixes fine and is clear. Used 1.8ML Bac water. Some of SSA reps seem confused telling people to use their WA-10 which is sterile water. I'm def not using sterile water for GH.

 

[CharlesV]

Some of SSA reps seem confused telling people to use their WA-10 which is sterile water. I'm def not using sterile water for

SSA should stick to selling peptides. it should be reconstituted with bacteriostatic water…but you already know that.

i have not heard a good explanation to why they advise sterile water? perhaps for plausible deniability when a bad vial is reported? “well, you are supposed to use our sterile water”?

 

[Jron]

SSA should stick to selling peptides. it should be reconstituted with bacteriostatic water…but you already know that.

i have not heard a good explanation to why they advise sterile water? perhaps for plausible deniability when a bad vial is reported? “well, you are supposed to use our sterile water”?

I thought maybe that could be what's going on. Using that line about how any cloudy GH is the users fault for not using their sterile water. But I think some of the sales reps are confused as to the difference between Bac and sterile water.

Their list even says BA-10 and WA-10 are both Bac. But if you look, it says BA-10 does contain alcohol and WA-10 does not. This is why I think they are confused cause of how both products are called Bac water. I got a 10 pac of their Bac water and will use it next time I mix a vial up to see if it's cloudy or clear.

 

[CharlesV]

But I think some of the sales reps are confused as to the difference between Bac and sterile water

that makes as much sense as anything i can come up with. first time i heard them advising sterile water, it had me spinning, checking all my notes to make sure i wasn’t crazy.

there is a language barrier too, which has caused some confusion that i’ve seen.