1st Filtering experience...TIRZ 20 mg w/2.0 BAC

[niqTM]

Is there a noticeable difference of loss solution in the 13mm vs 4mm when filtering 1ml of Tirz ?
Good to see you bro

 

[Jkeepdrop739]

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Just flush thru or pre soak filters. I do both.

 

[Ghoul]

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They only tested Tisch filters, the brand they sell.

Their overpriced 4mm filters hold up 0.07ml, while other brands of 13mm filters typically have a holdup volume of 0.03ml or less. Cobetter 13mm for instance holdup volume is 0.025ml. If you're reconstituting with 3ml, that's under 1% loss even if you don't bother purging.

 

[niqTM]

They only tested Tisch filters, the brand they sell.

Their overpriced 4mm filters hold up 0.07ml, while other brands of 13mm filters typically have a holdup volume of 0.03ml or less. Cobetter 13mm for instance holdup volume is 0.025ml. If you're reconstituting with 3ml, that's under 1% loss even if you don't bother purging.

good to know.i always went by that screen shot. I'll try cobetter filters.

 

[deadbeef]

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Pep Test doesn't know how to do an air purge. His holdup testing is invalid.

Cobetter 13mm for instance holdup volume is 0.025ml. If you're reconstituting with 3ml, that's under 1% loss even if you don't bother purging.

Those holdup specs are after an air purge. If you don't bother purging, the holdup will be much higher.

 

[Luke391]

I am new to filtering so, asking for critique frankly and also confirmation on things like using the luer lock needle on the smooth end of the filter.

Not sure what you mean about new step...just saying what i did.

I will probably use a 25mm on my 2ml vials of TIRZ to avoid a plug adding a new filter to complete the task....for the other substances will try a 13mm to see if they plug or pass the whole load without plug, then adjust technique going forward based on that.

with a 25mm filter you will loose 1ml of product

 

[Luke391]

A 13mm, even a 4mm syringe filter *should* be sufficient for a single vial of any peptide.

But I've encountered many that completely clog a 13mm filter before the entire vial is processed. A pretty good sign something's fucked up, but that's another topic.

As a practical matter 25mm will prevent this from happening, cost roughly the same, and the main concern, "holdup volume" or liquid trapped in the filter, the reason you choose the smallest needed, is inconsequential with a 25mm as long as you're reconstituting with 2ml+.

13mm filters will hold up 0.2ml, not 0.03ml.

 

[Elektrobot]

Surprised/not surprised when my 13mm filter became plugged solid (would not pass air or TIRZ for the last few drops ~.03ml or so...).

Is it possible that your new vial didn't have a vacuum and you got an air lock instead of the filter being plugged? The sterile vials I get aren't vacuumed and this happened to me the first time I tried it. I vent them with a needle now as I fill.

 

[BeardedGuy]

I find filtering to be kinda messy. Even with a vent needle. I do not like my results. The pressure difference always leaves me with a bubbled up vial stopper and an aspiration puff upon removal. I do it, it’s just tough for me.