Tirzepatide raw

[Kunu]

I'm new to this world, but it seems this might be the only place for answers to questions like this. If I have raw tirzepatide, how ridiculous is it to try to sterile fill on my own?

 

[Deadp007]

...you'd have to lyophilize it first
(which is just a nerdy way of saying "freeze-dry" it).

 

[Kunu]

Thanks. So lyophilization, then just sterile fill? Significant contamination risk, or simple and straightforward with some knowledge of principles of Chemistry?

 

[Raptor1]

Following. I would love know how to brew some of my own peptides.

 

[Magnus0u812]

I would be very cautious doing this. Having a sterile environment and process is a big deal.

 

[null88]

lyophilization machines aren't cheap

 

[maryslittlegoat]

lyophilization machines aren't cheap

Why is lyophilization necessary to make it usable? Raw is completely unusable, correct?

 

[Jire]

Why is lyophilization necessary to make it usable? Raw is completely unusable, correct?

I think heat will rapidly degrade the compound (unlike what I think about AAS), so you must sterilize in a different manner. Peptides are so fragile even shaking the bottle can degrade them.

 

[HB_22]

Why is lyophilization necessary to make it usable? Raw is completely unusable, correct?

If it is not lyophilized it wont do it's job after injecting it.

I think heat will rapidly degrade the compound (unlike what I think about AAS), so you must sterilize in a different manner. Peptides are so fragile even shaking the bottle can degrade them.

This is a myth and most peptides are very stable even when shaked.

 

[maryslittlegoat]

Without lyophilizing peptides will not have the same effect after reconstituting? Can you please elaborate why? People that do not know or understand these things (like me) are wasting a lot of $ and maybe serious associated safety risks.

Focusing on why it won’t work over safety seems the best way to get some people to wake the F up and start asking questions.

It’s scaring me honesty what I’m reading people are blindly doing in other forums.

 

[Jire]

If it is not lyophilized it wont do it's job after injecting it.


This is a myth and most peptides are very stable even when shaked.

Where can I learn more about lyophilization, homebrewing peptides, or their stability?

 

[clearheaded]

it will come already lyophilized obv, just will be in a vac bag vs vial. issue is u cant filter it like u can oils, and it won't keep like oils will. dont be cheap and just buy it pre vialed..

ie forget it no one home-brews peptides.... only reason to not get vialed GLP is if u are taking it orally. even then will be such a pain to work with to dose and keep 100% free from atm moisture

 

[Deadp007]

Pretty sure it does not comes pre-lyophilized.
But at any rate, I think you'd need a sterile room, well beyond the scope of 99.99% of citizens.
Maybe if you had Elon Musk type of money.... but then, why bother...

 

[maryslittlegoat]

There are China sources selling none lyophilized, non vacuumed vials of tirz. It is a thing. People are blindly buying it, reconstituting and injecting it.

No thank you. It is alarming.

 

[test9999]

There are China sources selling none lyophilized, non vacuumed vials of tirz. It is a thing. People are blindly buying it, reconstituting and injecting it.

No thank you. It is alarming.

I don't doubt this happens, along with all sorts of other alarming things. The question is how do we know who to trust. Sources testing everything prior to sale is all I can think of.
Example, I have a Chinese source selling 10mg Tirzepatide kits for $250
Do I take the chance? Haven't decided.

 

[maryslittlegoat]

So what’s ur understand why it has to be freeze dried? Sounds like its for shelf life for the stability of the peptide. Apparently there is ALSO something that it does so med delivers correctly in the system. Do you know what that is?

Ask if it is lyophilized. Ask if vacuum sealed. Ask if they have current labs. I bet they say no.

 

[clearheaded]

nothing to do with delivery of meds and everything to do with making it stable. 100% dry aka lyophilized is essential for stability, and why all peptides are. few are far more stable lik ghk-cu but still will have been freeze dried.

 

[maryslittlegoat]

nothing to do with delivery of meds and everything to do with making it stable. 100% dry aka lyophilized is essential for stability, and why all peptides are. few are far more stable lik ghk-cu but still will have been freeze dried.

Got it, thank you. If a peptide is unstable what could happen?

Initial thought is amino chains comprising the peptide break apart easily without being stabilized? If correct, when reconstituted an unstable peptide is likely to have the amino chains break apart. Making the peptide essentially useless. That’s a total guess though.

I do understand getting lyophilized peptides is absolutely essential. I’m not questioning that, just trying to understand further.

 

[clearheaded]

just ask QSC as likely can talk to there engineers and get a proper answer. I dont know, but armed with logic and some minor understanding.

my assumption is peptides degrade into there aminos and or variations of smaller chains ie smaller peptides. fold is another option re dimmer.

long and short of it is dont order bulk and attempt to home-brew peptides unless for topical application.

 

[buffaloindierock]

1. You don't need to lyophilize it. You can use a 0.3 micrometer syringe to filter. Read up on the best syringes because you want to also minimize peptide adhesion when doing this. Just measure and mix your raws with BAC and then use the filter syringe to transfer to a sterile vial.

2. If you do want to lyophilize it, it's because you want to make it more stable and long-lasting for dry storage. You don't need to buy a machine. Lots of universities have chem programs where you can take a training course to pay and rent the lab/equipment...and/or they allow students/research assistants to do it (it generates revenue). Ask about a sanitization step during lyophilization (it's not automatic - not all lyophilization is sterile).