Clover 0080
Member
Your just like and endless supply of information. Seriously I've learned more from this thread and specifically ghoul than the last 5 years of my life.
MESO-Rx
Anabolic Steroids
Qingdao Sigma Chemical Co., Ltd (International, US, EU, Canada and Australia domestic
- Thread starter Qingdao Sigma Chemicals
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Damned if you do, damned if you don't.
Flyfisher23
New Member
not prostate orgasms
Letits now
Member
Ugh. And on this note, I’m sticking around a little longer to see if T returns. I saw that Ray is back but many CNY festivities last until next week. So we’ll see. Doesn’t look good.
Looks like the 4000th page promo will never happen.
Swolemates365
Member
Like I said, I’ll give till 12th then I’m chalking up a 1k loss
Letits now
Member
Ouch. Thats a tough one to swallow. At least it would be for me. I know a lot of you guys are straight ballers. Rolexes and filters and so on.
MissedArmDay66
Member
Do any of you guys who are doing per dose filtering of peps/ hgh by back filling into slin have any pointers for how to do it without losing so much after plunger is reinserted and how to even get correct measure for lower doses. Even a 10 unit dose is difficult to accurately measure off the host 3ml syringe so provided the fluid goes to bottom of slin it can be measured that way but then for me I’m losing a lot reinserting plunger no matter how i hold the syringe and the peps won’t flow in the syringe like oils do. @SWFL-239 @Ghoul
Swolemates365
Member
Def not a baller. I was reselling local so it was a gamble from the start.
mok4315
Member
I’m on this struggle bus too. I’ve actually given up on this method, so hopefully ghoul has pointers. The things that make fluid loss marginally smaller are using a needle to back fill so fluid goes straight down without air bubbles, then slowly putting in the plunger until it’s barely secure, flipping the syringe so the fluid falls to the plunger end, then getting the air out. But it’s messy.
Ghoul
Member
It's probobly best demonstrated by a video, but I'm not sure I'm motivated enough to make one.
It did take some trial and error, making a few messes. but I'll give you a few tips that may help.
-I use the cap from the "push" end of the insulin syringe as a place to stick the plunger. It won't "stand up", but its a sterile place to put the rubber plunger into and lay it down.
-I use a 1-1.5" large gauge needle to avoid creating bubbles, stuck deeply into the syringe barrel, angled against the barrel wall, without completely blocking off the open end of the insulin syringe. This allows the liquid to flow to the bottom of the insulin syringe easily, without getting "caught", above a bubble, as you've likely experienced.
-Once finished filing, holding both syringes in one hand I recap the 3ml filtered syringe and put it down.
-Then I stick the insulin syringe plunger in *just barely". It's not even in enough to stay in on its own. It only closes off the open end and must be held there. Obviously you don't want to push out any liquid.
-Flip the insulin syringe needle side up (still capped btw), hold the barely in plunger to keep the open end closed.
-I flick the barrel with my fingernail to move the air bubble to the top. Once it's at the top, I can push the plunger in, pushing out the air (you can still leave the needle end cap on), and set down the perfectly dosed syringe for later or immediate use.
It's easier than it sounds once you practice a few times, Even small doses are no issue, though you should really be diluting to get more than .10ml volume for any peptide.
Maybe BD will sponser a video, lol.
Also, I realize this isn't "perfect", no technique is. I am improving this, eliminating the need for backfilling for per dose filtration, supplies are more expensive using this somewhat better technique. Another thing you should aim for, at least with the 3ml syringe that'll hold peptides for an extended time, is a silicone free syringe. Silicone, though common as a syringe lubricant, in increasingly understood to be bad to inject generally, but it has a negative effect on proteins/peptides in particular.
The other possibility, if you have really good control, is just to stick a zero dead space 31g needle on the end of the filtered 3ml syringe, then remove and dispose the needle after injecting. Like a "manual" injection pen. This adds a few complications too, but nothing insurmountable. It does add the risk of overdosing though and requires a very steady hand.
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This is the way for insulin syringes. Although I do not prefer them.
The cleanest way is to front load a leur lock syringe.
MissedArmDay66
Member
I’m using a 1 1/2” 18ga needle to push the fluid in and i think i finally got to the point where it’s going all the way down, its just getting the fluid to move back towards plunger so i have some air to push out is my biggest issue but ill stick with it lol. Definitely a lot of trial and error with this method
MissedArmDay66
Member
Really appreciate the info and thanks for adding the pics, i guess at this point for me it’s a finesse thing with the plunger. Right now i think im putting it in too much for fluid to flow back easily and the pressure is pushing out the fluid. I’m also having difficulty finding a source for the silicon free syringes so if you have one and don’t mind sharing i would appreciate it. I am storing the filter syringes upright with a bit of air between plunger and fluid to try and minimize contact.
As far as dilution and dosing goes i add as much bac water as i can. For example i have 10mg vials of both TB and BPC that are actually around 12 mg. I add 3ml of bac to both but for my desired dosing of 250-300 mcg x 2 day, i’m stuck with small number of units to dose. To dilute anymore i would have to either transfer to bigger vial or go with 5mg vials
Thanks for the help @Ghoul
lmao sorry idk why I did thatTag the gentleman who wrote about the seized package, though, not me
Thanks
synthesis30
Member
Wouldn't this significantly increase chances of contamination? Opening up sterile areas of the syringe and the peptide liquid to open air? For anyone into mycology, you know it's super easy for things to get contaminated in the open air. Unless you're using a flow hood. Maybe I'm missing something, but at least the way you're demonstrating it, it seems like you're trading one risk for another.
Spaceman Spiff
Subscriber
Agreed.
I am very anti backfilling.
I'm not worried about true contamination, but for someone who is constantly talking about filtering Peptides this makes 0 sense lol.
Ghoul
Member
An easy method to increase the dilution, and avoid the need for a larger vial, is to draw all the BAC water, let's say it's 5ml, inject 3ml into the peptide vial to reconstitute, draw the peptide back into the syringe, then attach filter, and you've got 5ml of your peptide.
Here's one US source for silicone free, non-reactive lab grade syringes, Norm-Ject. The part numbers for the various sizes are on the chart below. "Norm-Ject" are actually made by B.Braun, so, easily available in it-he EU from Altruan and other med suppliers.
Mongo966
Member
You banned bruh?Tag the gentleman who wrote about the seized package, though, not me
Thanks
Ghoul
Member
I saw this coming, and intentionally made the comment about an "imperfect" process, and that I had eliminated backfilling entirely, via a couple of incrementally better approaches. Add a 31g needle to the filtered syringe, if you're confident you can control it well enough to not inadvertently overdose, and any concern about backfilling is eliminated. Or
use a luer coupler, and "front fill" a zero dead space luer syringe.
Still, after some practice, I can do this very quickly now, holding the plunger in my hand, leaving the insulin syringe exposed for only a few seconds.
"OMG an exposed syringe opening you don't care about sterility."
EVERY SINGLE LUER LOCK. SYRINGE AND NEEDLE ARE OPEN AND EXPOSED TO A NON-STERILE ENVIRONMENT ONCE REMOVED FROM THE PACKAGE.
Look at these gaping holes.
Is it ideal to have an open syringe? No.
Yet luer needles and syringes are packaged separately, exposing both to a non-sterile environment once opened. In fact syringes are often filled with one needle (large gauge, blunt, or filter) which is then removed, again exposing an OPEN syringe and now the contents to a non sterile environment, and another needle is removed from its packaging, exposing its open end to a non-sterile enviornment, and then attached.
How about ampules, commonly used for injectable drugs by doctors. Are they exposed to a non-sterile environment briefly?
That's a far bigger opening than an insulin syringe, and takes the same or more time than backfilling .5ml.
The risk is low enough ampules and luer syringes are routinely exposed in clinical environments, even hospitals. Is that ideal? No, but then neither is you filing vials in a non-sterile environment, without a sterile flow hood, that you supply to other people, putting their health at risk.
Acting intelligently means understanding that while perfection is the goal, striving for it shouldn't be an impediment to incremental improvements where and when they can be implemented.
On balance, filtering removes existing bacteria, which Jano has found in peptides, particulates that are almost certainly present, and aggregates, the latter two are most dangerous with cumulative exposure. Any time exposed to a non-sterile environment risks the possibility of new post filtering bacterial contamination, but as a practical matter, based on the routine use of luer syringes and ampules, the risk is very low. Even the potential worst case outcome, unless you're somewhere with uniquely dangerous bacteria, creating a serious hazard, particularly in bacteriostatic solutions where bacteria cannot
replicate, is extremely minor.
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readalot
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