is here any Nobel price winner in microbiology ?

[mercury]

Is anybody using UV light to kill viruses and bacteria

I know that using wahtman filter is a good practice but what about leaving UV light on next to the vials

 

[Purge]

Reading the Wikipedia article, it seems like UV is a secondary method to disinfect. When used with water, you also have to filter out any particles big enough to block the light from hitting the bacteria. Since you're already filtering the gear, it's not clear the UV adds anything. Also, I couldn't find anything that said whether the UV might degrade the gear. Most steroid molecules are small and relatively stable, unlike DNA.

 

[03xx52]

I do not have a nobel prize but I am working on my phd in neuroscience. We do cell cultures and I am in charge of contamination mitigation, the biggest problem with UV and heat is they do not kill endospores. An endospore is a state bacteria go into when they are in a threatening environment and although heat (>100C) normally denatures most proteins the endospore is largely unphased. UV will cause what is known as a thymine dimer to form, DNA mutation, on bacteria. Bacteria that form endospores (most bacillus and some others) are not effected by this UV. The only way to truly sterilize something is in an autoclave, you can get very close with a dry over at 170 C for over an hour. Chemical sterilization is another way.....Filter works great as well for bacteria but not viruses...Viruses hate heat.....
I would filter, even dead bacteria can cause harm (endotoxins)...filtering will remove most bacteria.
Sorry for the long reply

 

[03xx52]

One more thing the UV will be blocked by the vials glass.

 

[Big_paul]

looks like we found one, but if you cant understand what this brother has said. heat your gear 15min at 275 degrees F. do this 3x. let cool to room temp before you re heat. you will be safe and still have some cash left in your pocket.

 

[03xx52]

Yeah heat then filter. If you want to get crazy heat then filter then cool three times.