Dry Heat Sterilization

Borderline

New Member
Hello friends
Is the dry heat sterilization method a correct method to sterilize vials?
Will this dry heat sterilization machine work for this method?
Thanks

2_org_zoom.jpg
 
Unfortunately if you don't give us any specs on it like the temperature it reaches and things like that we can't give you an answer maybe post a link to where you're getting it from so I can read up on it and let you know but I can also tell you as long as you have a clean oven at home you can use that for dry heat sterilization no problem 350゚ for 45 minutes
 
Sorry i didnt add link because of turkish vendor page.
It has 50-250•C
This will work for sterilizing your vials. However it is not tall enough to really fit a filter funnel or a filtering flask inside. It's 9.8" wide I'm sorry I has to converted to inches so you might be able to get a filtering Flask inside but definitely not a filter funnel. I see there is 2 different ones on there i just didn't have the time to look at the 2nd one maybe it's a little bigger because I would say a filtering flask 1000ml is about 10" tall but a glass filter funnel is about 12 to 13" tall. Then again I don't know what you're using to filter. You could be using a peristaltic pump and capsule filter. Then again OP I guess your question was will it work for sterilizing vials the answer to that question is yes.

I wouldn't mind asking you a question about it if you could send me a PM
 
Unfortunately if you don't give us any specs on it like the temperature it reaches and things like that we can't give you an answer maybe post a link to where you're getting it from so I can read up on it and let you know but I can also tell you as long as you have a clean oven at home you can use that for dry heat sterilization no problem 350゚ for 45 minutes
I usually do 450 for my beaker.

But 450 at that amount of time would work?
 
I usually do 450 for my beaker.

But 450 at that amount of time would work?
That's a little aggressive don't you think considering this is from cdc.gov website

The most common time-temperature relationships for sterilization with hot air sterilizers are 170°C (340°F) for 60 minutes, 160°C (320°F) for 120 minutes, and 150°C (300°F) for 150 minutes.
 
That's a little aggressive don't you think considering this is from cdc.gov website

The most common time-temperature relationships for sterilization with hot air sterilizers are 170°C (340°F) for 60 minutes, 160°C (320°F) for 120 minutes, and 150°C (300°F) for 150 minutes.
Never tried vials. But geez that would be amazing. I believe xkawnx did something similar. Do you soap, alcohol, and distill water?

Does anyone else know?
 
I remember reading someone (mentioned above) did

Dawn soap, distilled water, then rubbing alcohol, then 450 for 15-30 min. But i took this method to me pre filtered beaker.

But this was used for vials. When mentioned

I would think it works.
 
Never tried vials. But geez that would be amazing. I believe xkawnx did something similar. Do you soap, alcohol, and distill water?

Does anyone else know?
Personally I put Is my distilled water into a Big Tubaware container, Then dip the vials or beaker or whatever we're talking about into the distilled water, then spray it with the iso alcohol as I keep that in a spray bottle. It doesn't need to be soaked in the alcohol. It will Dry just fine being sprayed and you can work much faster. The other reason I spray it and this is just personal preference instead Completely Saturating my vials in the iso, and I've never had this happen but I've heard stories about the alcohol sticking to the side of the vials during dry heat sterilization and then being a possible culprit for PIP down the road. I'm not saying this will happen as I've never had it happen but Someone who was knowledgeable said this to me years ago and for that reason I've always sprayed it.
 
You guys need to think about the alcohol step and what you're trying to achieve...

Unnecessary
But I'm personally trying to get the vial to come out completely dry they looked out spraying it was alcohol juggernauts oh you really have to leave it in there that's really hard for 50゚F for about 2 hours.

If you know something I don't, I'm always willing to learn?
 
Since you are sterilizing with heat, alcohol serves no purpose on the sanitary side of things, just an extra step.
It's not about sanitization when it comes to the alcohol. It's about the vial drying while it's in the dry heat sterilizer
Because without The alcohol you can put it in there and @ 350゚F for 45 minutes but it's still gonna be wet when you take it out .

I know how long you've been on the forums for got me a person I've been on them since 2012 and it used to be a place where you answered questions and try to help people out that need help learning. What I'm getting that is instead of just making a statement that alcohol does nothing for the sanitary side of things which we already know, and we're talking about sterilization not sanitization. Why don't you elaborate and explain to OP What you mean by that or explain what you think is the proper way to do it in order to help... Also I say that with no Disrespect.or to be ignorant. So please do not take it that way. Thanks brother...


You guys need to think about the alcohol step and what you're trying to achieve...

UnnecessaryI don't know about you but I'm more than here to help other people who are still learning. I don't know how long you put on forms for but But remember If you remember before probably 2016 everyone was just trying to help each other out...
 
Since you are sterilizing with heat, alcohol serves no purpose on the sanitary side of things, just an extra step.
Like I just told @Drugsarefun Instead of just making a statement why don't you elaborate and try to help OP, and other people who are trying to learn as to why you say alcohol serves no purpose as I can learn to you from over 12 years experience it damn sure does. Unless you're using a autoclave with a drying function. Also as I just told him I mean this with no disrespect. Even though it might come off like I'm being a smartass, trust me I'm definitely not I'm not that kind of person. I have no fun but respect for everyone trying to learn.
 
Like I just told @Drugsarefun Instead of just making a statement why don't you elaborate and try to help OP, and other people who are trying to learn as to why you say alcohol serves no purpose as I can learn to you from over 12 years experience it damn sure does. Unless you're using a autoclave with a drying function. Also as I just told him I mean this with no disrespect. Even though it might come off like I'm being a smartass, trust me I'm definitely not I'm not that kind of person. I have no fun but respect for everyone trying to learn.
Yes, sorry looks like I may have miss understood what you're trying to achieve, although I'd say 90% of the people using alcohol would be doing so so they can be "extra clean"
In which case it's a waste of time before your vials are sterilised as sterilising will kill everything if done correctly.
I've never heard of someone doing it for drying purposes, I don't have this issue but can see the benifit.
 
Yes, sorry looks like I may have miss understood what you're trying to achieve, although I'd say 90% of the people using alcohol would be doing so so they can be "extra clean"
In which case it's a waste of time before your vials are sterilised as sterilising will kill everything if done correctly.
I've never heard of someone doing it for drying purposes, I don't have this issue but can see the benifit.
You have never had an issue with your vials coming out wet from washing before you put them in? If that's the case I'm assuming you're using a autoclave with a drying function and not any other kind of eating element such as a household oven or a lab oven because 99% of people will tell you unless sterilizing it at 400° for 2 hours which is way too long considering it is only called for $350° f 45 minutes but if you do it like that 99% of people will tell you the vials will come out still wet. Which is why some people dip them in alcohol personally I spray the inside with a mist of alcohol so that it will dry in the 45 minutes that it's in the oven and the extra 20 minutes or more that it takes to cool down
 
You have never had an issue with your vials coming out wet from washing before you put them in? If that's the case I'm assuming you're using a autoclave with a drying function and not any other kind of eating element such as a household oven or a lab oven because 99% of people will tell you unless sterilizing it at 400° for 2 hours which is way too long considering it is only called for $350° f 45 minutes but if you do it like that 99% of people will tell you the vials will come out still wet. Which is why some people dip them in alcohol personally I spray the inside with a mist of alcohol so that it will dry in the 45 minutes that it's in the oven and the extra 20 minutes or more that it takes to cool down
I've made a post with my process once before, although it doesn't include the cleaning stage, which would be warm soapy water, then a distilled water rinse x 2, into the oven for 20mins to dry, then into autoclave pouches for the pressure cooker, 15psi for 45mins, no condensation on the vials, no use of alcohol.
 
I've made a post with my process once before, although it doesn't include the cleaning stage, which would be warm soapy water, then a distilled water rinse x 2, into the oven for 20mins to dry, then into autoclave pouches for the pressure cooker, 15psi for 45mins, no condensation on the vials, no use of alcohol.
I'm starting to get a feeling that people get different results using the same method based on the amount of moisture present in the surrounding air as there is no way to prevent air getting in the vials when you move them from oven to autoclave.
now will this tiny amount of air contain enough moisture to cause an issue I have no idea....
 
I'm starting to get a feeling that people get different results using the same method based on the amount of moisture present in the surrounding air as there is no way to prevent air getting in the vials when you move them from oven to autoclave.
now will this tiny amount of air contain enough moisture to cause an issue I have no idea...


why would your vials be going from the oven to the autoclave? You're going to either sterilize them in the oven using dry heat sterilization at 350°F for 45 minutes or in a autoclave at 15 psi and 121 ° C but they shouldn't be going from the oven too the autoclave.
 

Sponsors

Back
Top