Have any NAD+ that was unusable because it was unbuffered, and therefore like injecting lemon juice? Well with this guide you can turn that into a superior product with virtually zero discomfort.
I’m going to explain what I did and you can adapt the steps to your own set up as needed.
You will need:
1 30mL bottle bacteriostatic water.
sodium phosphate monobasic
sodium phosphate dibasic
pes sterile syringe filters 0.2micron
5-10ml Syringes
27g or similar needles
0.001 Milligram scale
Weigh boats ideally
Sterile 5-10ml vials
Lyophilised NAD+ (in my case it was 600mg.)
I recommend hydrated forms of these salts as it makes the masses more accurate as anhydrous versions can absorb varying amounts of moisture from the air, making the calculations less accurate.
Whichever form you get, you can use an AI tool to calculate the masses of each salt needed. You want to create an isotonic buffer (with no added osmotic agent such as NaCl) at 6.5pH with 30mL of water so just ask it for that and specify the exact forms you have.
I used anhydrous anyway (didn’t think of the moisture issue when I ordered it) so that was about 420mg of monobasic and 100mg of dibasic.
I weighed this with a milligram scale and a weigh boat. I then added the powder to the inside of a syringe barrel with a needle attached, and drew water from the bac water bottle into the syringe to dissolve the powder.
I then emptied the syringe into the bac water bottle and let it mix thoroughly.
This is the buffer ready, so use it to reconstitute a vial of nad+. In my case I needed 6ml to buffer 600mg of nad+ you may need more or less depending on how much nad+ you have per vial. Seems reasonable to assume 1ml per 100mg.
Use the 3ml vial and the syringe to hold the amount of buffer you need.
Prepare your filter by rinsing a ml or two of normal bac water through it before adding a needle to the end. Then swab your sterile vial with an alcohol swab, allow to dry, insert a 27-30g vent needle off centre in the stopper and then insert the filter needle on the opposite side of the vial also off centre.
Insert your syringe into the filter ensuring no air bubbles get trapped in the filter as these will greatly slow the pace of filtration. You can pull back on the plunger if any get stuck to draw them out and back into the syringe.
You’ll want to do this every time as the bac water buffer you made isn’t sterile. I think this should be fine given it contains benzyl alcohol and you will be filtering it.
However on reflection I might recommend to the reader to be on the safe side:
Filter the phosphate salts into the bac water bottle instead of just adding it straight. This would protect against any theoretical microbe growth that might happen in the bottle.
Anyway, after doing this a moment ago and injecting some to try it out, I can confirm it works with basically zero discomfort subcutaneously.
I’m going to explain what I did and you can adapt the steps to your own set up as needed.
You will need:
1 30mL bottle bacteriostatic water.
sodium phosphate monobasic
sodium phosphate dibasic
pes sterile syringe filters 0.2micron
5-10ml Syringes
27g or similar needles
0.001 Milligram scale
Weigh boats ideally
Sterile 5-10ml vials
Lyophilised NAD+ (in my case it was 600mg.)
I recommend hydrated forms of these salts as it makes the masses more accurate as anhydrous versions can absorb varying amounts of moisture from the air, making the calculations less accurate.
Whichever form you get, you can use an AI tool to calculate the masses of each salt needed. You want to create an isotonic buffer (with no added osmotic agent such as NaCl) at 6.5pH with 30mL of water so just ask it for that and specify the exact forms you have.
I used anhydrous anyway (didn’t think of the moisture issue when I ordered it) so that was about 420mg of monobasic and 100mg of dibasic.
I weighed this with a milligram scale and a weigh boat. I then added the powder to the inside of a syringe barrel with a needle attached, and drew water from the bac water bottle into the syringe to dissolve the powder.
I then emptied the syringe into the bac water bottle and let it mix thoroughly.
This is the buffer ready, so use it to reconstitute a vial of nad+. In my case I needed 6ml to buffer 600mg of nad+ you may need more or less depending on how much nad+ you have per vial. Seems reasonable to assume 1ml per 100mg.
Use the 3ml vial and the syringe to hold the amount of buffer you need.
Prepare your filter by rinsing a ml or two of normal bac water through it before adding a needle to the end. Then swab your sterile vial with an alcohol swab, allow to dry, insert a 27-30g vent needle off centre in the stopper and then insert the filter needle on the opposite side of the vial also off centre.
Insert your syringe into the filter ensuring no air bubbles get trapped in the filter as these will greatly slow the pace of filtration. You can pull back on the plunger if any get stuck to draw them out and back into the syringe.
You’ll want to do this every time as the bac water buffer you made isn’t sterile. I think this should be fine given it contains benzyl alcohol and you will be filtering it.
However on reflection I might recommend to the reader to be on the safe side:
Filter the phosphate salts into the bac water bottle instead of just adding it straight. This would protect against any theoretical microbe growth that might happen in the bottle.
Anyway, after doing this a moment ago and injecting some to try it out, I can confirm it works with basically zero discomfort subcutaneously.
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