Chinese HGH - Clear or Cloudy

[OldGHGuy]

Not to hijack this thread but I've got a similar issue at the moment....one of these vials is 100% jano tested and the cloudy one I've stuck a lable on is from a local guy....
I think I have to ask him how he stored it.
I can't even draw it through a 29g insulin needle

Yuck. That's beyond "cloudy". I wouldnt even think about using that

 

[ilosaari]

Odds are the amount of aggregated, inactive rHGH, unless it looks like milk, isn’t more than 10%, more likely low single digits. Worst case scenario I’ve seen are scientists using very high heat getting about 30% loss via aggregation. Since most are overdosed I don't think any dose adjustment needs to be made.

As far as “is it ok to inject after filtration?”.

For my risk tolerance yes.

Aggregates need to fall within a certain “looks like bacteria!” size range for the immune system to react and cause immunogenicity.

Thats 0.1 µm to 10 µm.

Aggregates must be at least 100um in size to be visible by the way, so relatively huge. (Those large aggregates cause other issues, but I’m focused on immunogenicity as the biggest threat).

So a .22um filter is removing 99% of the aggregates in the immunogenicity triggering size range. The small amount left is probably on par with the levels found in pharma (or even less, to be honest).

This experiment was conducted by one of the worlds leading experts on peptide pharmaceutical development to demonstrate why even pharma peptides / proteins should be filtered, in part, to avoid immunogenicity causing drugs to become less effective:

Here’s the result of pharma* rHGH filtered with .22um PES syringe filters, the same type I use.

From 37,000 >1um sized particles / ml down to about 300.

View attachment 357594

This was expired rHGH, and shaken, to simulate worst case scenario of what a patient might be exposed to with poorly stored and handled pens. Pretty sure it’s safe to assume UGL is always “worst case scenario” condition.

So knowing filtering completely neutralizes the aggregate threat, and the cumulative harm from all the other shit found in cheap vials being injected on a daily basis,

View attachment 357596View attachment 357595

along with guaranteeing sterility since Jano found a high proportion of peptide vials containing bacteria, a shock, I know, when most are likely produced in peptide labs like this one:

View attachment 357597

that doesn’t make UGL peptides risk free, but lowers it enough to make it tolerable for me.

There are a number of them, none are that great but you’ll get the basics and can develop your own technique from there. Consider a practice run pretending an empty vial is lyophilized, so you don’t end up wasting a vial due to some accident.

This essentially covers it. I use 3ml syringes, and a single 25g 1.25” needle for all steps. A large gauge needle is preferable to keep pressure, and therefore shear stress low, which can damage some peptides.



View: https://youtu.be/fmz4241qPn4

Any good source for sterile vials? Like good quality sterile vials

 

[Ghoul]

Any good source for sterile vials? Like good quality sterile vials

These are the best available to the general public. Certified to comply with USP (pharma) standards. Ie, particulate free etc.

Ultra Spec Sterile 2ML (13mm neck) Clear Sealed Glass Vials

In order to receive vials in sealed shrink wrap boxes, please order in multiples of 25 Ultra Spec Sterile 2ML-13mm Clear Sealed Glass Vial, Qty 1 ULTRA SPEC sterile depyrogenated vials are assembled with Teflon coated non-latex chlorobutyl rubber stoppers (conforming with USP<381>) and vials...

www.medical-and-lab-supplies.com

The stoppers are teflon coated, preventing chemicals from the butyl rubber from leeching into the solution. This not only saves you from regularly injecting small amount of that toxic, potentially carcinogenic soup, those chemicals have been demonstrated to degenerate peptide / proteins.

 

[ilosaari]

These are the best available to the general public. Certified to comply with USP (pharma) standards. Ie, particulate free etc.

Ultra Spec Sterile 2ML (13mm neck) Clear Sealed Glass Vials

In order to receive vials in sealed shrink wrap boxes, please order in multiples of 25 Ultra Spec Sterile 2ML-13mm Clear Sealed Glass Vial, Qty 1 ULTRA SPEC sterile depyrogenated vials are assembled with Teflon coated non-latex chlorobutyl rubber stoppers (conforming with USP<381>) and vials...

www.medical-and-lab-supplies.com

The stoppers are teflon coated, preventing chemicals from the butyl rubber from leeching into the solution. This not only saves you from regularly injecting small amount of that toxic, potentially carcinogenic soup, those chemicals have been demonstrated to degenerate peptide / proteins.

Thanks. I’m in Europe so its a bit expensive to order those, but not impossible.
Do you know if it’s possible to store peptides in polypropylene vial? I found a sealed injection sterile vial but is polypropylene