Sterility

[mongo5165]

IV tubing.

KSTek uses PTFE coated stoppers so you should be gtg.

How long do the butyl rubber stoppers last for AAS oils before chemical leaching is a concern?

 

[Sampei]

IV tubing.

KSTek uses PTFE coated stoppers so you should be gtg.

I'm not Sure about that I believe their vial use simple butyl stoppers

 

[Photon]

I'm not Sure about that I believe their vial use simple butyl stoppers

They advertise as PTFE but nobody really knows lol
The rubber feels kinda cheap tho.
I need the stoppers used by Pfizer in their bac water.
It's dense enough to bend needles lol

 

[Sampei]

They advertise as PTFE but nobody really knows lol
The rubber feels kinda cheap tho.
I need the stoppers used by Pfizer in their bac water.
It's dense enough to bend needles lol

Yeah their stoppers are like butter lol
Cheap as fuck.

Same for the sterile water here, fucking stopper are so thick last time I tried to pierce it with a slin pin it bended and break inside halfway xD

 

[RocketSurge0n]

IV tubing.

KSTek uses PTFE coated stoppers so you should be gtg.

@Photon how long is a vial good for once punctured? Doesn’t the expiration date move up a lot one a vial is punctured?

 

[Photon]

@Photon how long is a vial good for once punctured? Doesn’t the expiration date move up a lot one a vial is punctured?

I mean if you follow FDA standards its usually 30d or less.
But in reality it's not clear cut.

Type of vial, type of stopper, type of oil, oxidation level of oil, raw purity etc.
Personally I'd be comfortable using the stuff i brew for up to a year or two.

 

[Ghoul]

@Photon how long is a vial good for once punctured? Doesn’t the expiration date move up a lot one a vial is punctured?

28-30 days is the "rule of thumb" because of the possibility of introducing bacteria into the vial, which increases with every piercing. The FDA is using worst case scenario assumptions.

But 1 piercing is not going to be the same risk as 30 piercings.

In my opinion, if the vial constrains a preservative, like Benzyl Alcohol, as long as each time you sanitize the top with an alcohol pad, let it dry, and only use a new needle you can ignore the 30 day limit.

If there's no preservative, like sterile water or something reconstituted with sterile water (that doesn't have a preservative in the powder like metacresol), it should really be used within 48 hours (FDA and EMA says 24), and must be refrigerated. Reconstituted proteins/peptides are like raw egg yolk when there's no preservative. If any bacteria gets in the vial it will feed on it and quickly grow. If it's UGL, the bacteria is probably already in there, deactivated by lyophilization, ready to come back to life once water is added.

 

[RocketSurge0n]

28-30 days is the "rule of thumb" because of the possibility of introducing bacteria into the vial, which increases with every piercing. The FDA is using worst case scenario assumptions.

But 1 piercing is not going to be the same risk as 30 piercings.

In my opinion, if the vial constrains a preservative, like Benzyl Alcohol, as long as each time you sanitize the top with an alcohol pad, let it dry, and only use a new needle you can ignore the 30 day limit.

If there's no preservative, like sterile water or something reconstituted with sterile water (that doesn't have a preservative in the powder like metacresol), it should really be used within 48 hours (FDA and EMA says 24), and must be refrigerated. Reconstituted proteins/peptides are like raw egg yolk when there's no preservative. If any bacteria gets in the vial it will feed on it and quickly grow. If it's UGL, the bacteria is probably already in there, deactivated by lyophilization, ready to come back to life once water is added.

I mean if you follow FDA standards its usually 30d or less.
But in reality it's not clear cut.

Type of vial, type of stopper, type of oil, oxidation level of oil, raw purity etc.
Personally I'd be comfortable using the stuff i brew for up to a year or two.

Thanks guys. Much appreciated. I’m looking to start brewing for the first time and it seems that for small batches this method makes the most sense. Can either of you point me to your personal favorite beginners guide?

 

[nilescrypt]

Serious question, I work at a pharmacutical company, in particular with peptides.
I am kinda intrigued to start homebrewing stuff but there are a lot of questions from my end.
One of the biggest ones, it would probably be possible to use a 50ml syringe and a syringe filter to be clean but how are you being sterile? We use labratory hoods in clean rooms and A LOT of disinfectant.
But at home I dont have anything able to work sterile, I know there are "Bags" that get blown up with filtered air in which you can but your hands into.
Do you guys use somethin like that or just fuck it?

i caviwipe my workspace & lay down dental bibs

 

[RocketSurge0n]

i caviwipe my workspace & lay down dental bibs

Are you filling pre sterilized and capped vials or are you doing the capping?

 

[nilescrypt]

Are you filling pre sterilized and capped vials or are you doing the capping?

pre sterilized vials, it seems like too much of a hassle to do otherwise

 

[RocketSurge0n]

pre sterilized vials, it seems like too much of a hassle to do otherwise

How long are you willing to keep the vials around after making them up? Would you say they have the same expiration as un-pierced vials?

 

[gainslol]

How do you guys recap? With a laminar flow hood to prevent any dust from getting in? Or like a dust box?

Found these for $100:

 

[Andrewjo]

I picked up a laminar flow hood on govdeals. Been loving it for all lab activities

 

[Gonz0]

I mean if you follow FDA standards its usually 30d or less.
But in reality it's not clear cut.

Type of vial, type of stopper, type of oil, oxidation level of oil, raw purity etc.
Personally I'd be comfortable using the stuff i brew for up to a year or two.

Forgive my ignorance , but if someone were to filter a kit of test into a 100ml vial for long term storage , would they need to get an uncapped 100ml vial to transfer into or could they get an already capped 100ml vial and just puncture the top once and put a cap back on it ? Sorry if this is a dumb question, I want to filter my china oils and also make a blend of all the oils I’m going to use for my upcoming blast and on the lab supply site they have uncapped and capped vials. I’m also assuming the vials that come pre capped don’t have the type of stoppers that you guys are talking about for long term storage.

 

[Infernalmango]

Forgive my ignorance , but if someone were to filter a kit of test into a 100ml vial for long term storage , would they need to get an uncapped 100ml vial to transfer into or could they get an already capped 100ml vial and just puncture the top once and put a cap back on it ? Sorry if this is a dumb question, I want to filter my china oils and also make a blend of all the oils I’m going to use for my upcoming blast and on the lab supply site they have uncapped and capped vials. I’m also assuming the vials that come pre capped don’t have the type of stoppers that you guys are talking about for long term storage.

Sterile Barrier Vial Seals, Tamper Indicating Seal, 20mm, Silver, 20 Seals

Sterile Barrier Vial Seals, Tamper Indicating Seal, 20mm, Silver. Allows you to safely travel with your insulin or other type vials. Stores 2 Glass Serum Vials. Up to 12 hours of cooling time.

www.medical-and-lab-supplies.com

Only stabbing the stopper twice to filtering oil in. Then throw one of these bad chickens on. Store away.

 

[Gonz0]

Sterile Barrier Vial Seals, Tamper Indicating Seal, 20mm, Silver, 20 Seals

Sterile Barrier Vial Seals, Tamper Indicating Seal, 20mm, Silver. Allows you to safely travel with your insulin or other type vials. Stores 2 Glass Serum Vials. Up to 12 hours of cooling time.

www.medical-and-lab-supplies.com

Only stabbing the stopper twice to filtering oil in. Then throw one of these bad chickens on. Store away.

Much appreciated brother !