MESO "Specialized" Testing Group Fund

[Sera]

Janoshik Analytical

Here is the hplc for that sample

 

[Sampei]

Janoshik Analytical

Here is the hplc for that sample

HPCL of my sample

 

[readalot]

Request the "raw data" for HPLC data so we have it for posterity. Other peaks on the chromatogram? Surely a couple of the ones seen on the GC trace should show up (gonane cores)?

Since we are finding free "hormone" on some of the GC data where is the fatty acid if the ester is thermally cracking in the GC? Hint: it ain't.

 

[Sera]

Is the raw data the ‘ms’ in the hplc-ms?

 

[readalot]

Is the raw data the ‘ms’ in the hplc-ms?

No. Raw data from the HPLC chromatogram. LC trace with peak integrations. 5 bucks with order or 20 after.

GCMS is separate test. It comes standard with "raw" data.

 

[Sera]

yeah but there is a mass spectrometry available for hplc as far as I’m aware, and previously Jano has said they include it by default now for all hplc.

 

[readalot]

yeah but there is a mass spectrometry available for hplc as far as I’m aware, and previously Jano has said they include it by default now for all hplc.

His default detector is UV-VIS IIRC for standard UHPLC screenings. MS is an option but not standard.

Post in thread 'Readalots Enhanced Testing'

Nov 9, 2024

There isn't going to be any significant difference between the test ester and the test ester impurities on volatility or ionization in the MS.

Correct, I agree, but we can't be (yet) 100% sure whether the changes are not induced by the heat from the GCMS causing loss of hydrogen.

UHPLC to the rescue?

HPLC vs UHPLC - How to Choose?

How to choose between HPLC or ultra-high-performance liquid chromatography (UHPLC) as they each have their own advantages and disadvantages

www.ssi.shimadzu.com

The HPLC used for anabolic steroids is actually UHPLC (Shimadzu as well!).

Generally, in the industry you don't differentiate the two, UHPLC (and UPLC) are...

• janoshik

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[Sera]

@janoshik I thought I read somewhere that you include MS with all HPLC or is that only for specific tests?

Also, could LC-MS quantitatively identify some of these closely related substances maybe in these steroid samples or in any way be helpful to us here?

 

[dinfar1337]

His default detector is UV-VIS IIRC for standard UHPLC screenings. MS is an option but not standard.

Post in thread 'Readalots Enhanced Testing'

Nov 9, 2024

There isn't going to be any significant difference between the test ester and the test ester impurities on volatility or ionization in the MS.

Correct, I agree, but we can't be (yet) 100% sure whether the changes are not induced by the heat from the GCMS causing loss of hydrogen.

UHPLC to the rescue?

HPLC vs UHPLC - How to Choose?

How to choose between HPLC or ultra-high-performance liquid chromatography (UHPLC) as they each have their own advantages and disadvantages

www.ssi.shimadzu.com

The HPLC used for anabolic steroids is actually UHPLC (Shimadzu as well!).

Generally, in the industry you don't differentiate the two, UHPLC (and UPLC) are...

• janoshik

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• 

Big difference! without ms and only uv vis we cant identify unknown peaks! and it overestimates the purity.

with the hplc-ms we can identify each peak with the mol weight and match it to database.

how much extra did you say the ms option cost?

 

[readalot]

Big difference! without ms and only uv vis we cant identify unknown peaks! and it overestimates the purity.

with the hplc-ms we can identify each peak with the mol weight and match it to database.

how much extra did you say the ms option cost?

I didn't. It is on here somewhere.

 

[readalot]

Post in thread 'Janoshik Analytical laboratory testing services'

Oct 15, 2023

Hi. Just curious why your GCMS service is not quantitative?

Because we already have quantitative HPLC methods developed. Why develop (lot of time and $) and maintain for GCMS too?

Also GCMS is inherently less precise in quantitative analysis than HPLC.

Where can I find the assays/methods used for qualitative and quantitative AAS tests advertised on your website?

It's HPLC-DAD gradient methods, some details are here: https://janoshik.com/details/

ICP-OES for metals?


Thank you.

ICP MS

Cheers

• janoshik

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Here is the standard method: HPLC-DAD.

 

[dinfar1337]

I didn't. It is on here somewhere.

just trying to get spoon fed since i haven't seen it before. only gcms

 

[readalot]

Big difference! without ms and only uv vis we cant identify unknown peaks! and it overestimates the purity.

with the hplc-ms we can identify each peak with the mol weight and match it to database.

how much extra did you say the ms option cost?

Post in thread 'Source QC and C of A (do you have one?)'

Jan 22, 2024

Thank you for your time.

Your HR LCMS is dedicated to gh/peptide testing, correct?

1. So you have indicated your GCMS is set up to screen for AAS side products/androstane raw materials/other assorted organics. Understood. Cost: 170 USD

2. ICP-MS expandable as needed. Cost: 120 USD

3. Residual solvent analysis not available via preferred GC methods since you would need significant investment on the injection system for lower boilers. Got it. Can you provide a little more detail on the moisture analysis? Karl Fischer? Loss on drying? Cost TBD. Need to understand what this...

• janoshik

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See front page. Not sure it is set up for AAS. Above he says it is an option. Might be an interesting angle. Would need to consult with their team.

https://janoshik.com/pricing/

 

[readalot]

just trying to get spoon fed since i haven't seen it before. only gcms

I understand.

 

[dinfar1337]

See front page. Not sure it is set up for AAS.

gcms is defiently better for aas. only reason to run hplc ms is just for overall cost savings instead of running hplc-dad + gc-ms

 

[readalot]

and it overestimates the purity.

This is my concern and question I have had for a while now.

 

[dinfar1337]

This is my concern and question I have had for a while now.

so far we have 90% hit rate with the gcms only the tren e raws from kain werent bad. seems like a great investment for raws for us homebrewers.

ofc ugl is gonna save the money since nobody is putting pressure on them. valid concern!

 

[readalot]

gcms is defiently better for aas. only reason to run hplc ms is just for overall cost savings instead of running hplc-dad + gc-ms

If you want gnat's ass accuracy and ID of everything you need GCMS plus derivatization. We are here spinning our wheels between the HPLC and GCMS data. Co-eluting HPLC peaks? HPLC overestimating purity? Hard for me to reconcile the GCMS data with the HPLC purity data. Volatility and ionization of each component, etc. All over the internet LMAO.

Risk of false positive purity with current HPLC setup?

 

[dinfar1337]

Risk of false positive purity with current HPLC setup?

seems likes the number is way above 60% of all raws(low estimate).

the studies with profesionals looking into ugl gear is 100% concerned with this and probably not bad brews.

 

[Photon]

gcms is defiently better for aas. only reason to run hplc ms is just for overall cost savings instead of running hplc-dad + gc-ms

Issue is that GCMS wont give you purity% to brew oils.
Non-volatile stuff also won't turn up, so even if you have a nice GCMS, you might get low purity% due to non-volatile crap contamination.