Lobster HGH no vacuum - fridge vs no fridge test

[Sampei]

Yo! It's time bitches!

@TheLobster is gonna sponsor the test of the vials with no vacuum kept half in the fridge and half outside in a cabinet for the last 6+ months.

You ready?

 

[readalot]

Yo! It's time bitches!

@TheLobster is gonna sponsor the test of the vials with no vacuum kept half in the fridge and half outside in a cabinet for the last 6+ months.

You ready?

It's been 6 months. Damn.

 

[Ghoul]

Does anyone involved actually know the type of degradation that occurs when rHGH is oxidized?

Hint: It's not "purity". If you're not testing for what actually occurs, you're not going to see it.

 

[readalot]

Paging Nb2 cell assay. Who wants to scope, identify lab, and contribute?

 

[Ghoul]

Paging Nb2 cell assay. Who wants to scope, identify lab, and contribute?

Since this "lack of vacuum vial" is an experiment in progress, here's a more practical contribution. A quick protocol to measure what lack of vacuum / oxidation does to rHGH, that is, cause post reconstitution instability that needs to be evaluated over the course of several days post reconstitution. Two studies attached that clearly demonstrate how this happens to rHGH, and why pharma uses an inert gas to prevent lyophilized rHGH from being exposed to oxygen.

In plain english, if your rHGH vial lacks vacuum, the air that's now in the vial (20% oxygen), oxidizes the methionine in the rHGH, making it less durable and degrade (unfold and aggregate) faster once reconstituted than if it was vacuum sealed.

If you reconstitute and immediately measure purity, there probably won't be much of difference noticed.

 

[Sera]

Does anyone involved actually know the type of degradation that occurs when rHGH is oxidized?

Hint: It's not "purity". If you're not testing for what actually occurs, you're not going to see it.

Amount of hgh should decrease, if it oxidises then the chemical make up changes and therefore no longer is hgh

 

[readalot]

Does anyone involved actually know the type of degradation that occurs when rHGH is oxidized?

Hint: It's not "purity". If you're not testing for what actually occurs, you're not going to see it.

1991: reverse phase HPLC

The effects of formulation variables on the stability of freeze-dried human growth hormone - PubMed

Formulation often has a dramatic effect on degradation of proteins during the freeze-drying process as well as impacting on the "shelf-life" stability of the freeze-dried product. This research presents the results of a formulation optimization study of the "in-process" and shelf-life stability...

pubmed.ncbi.nlm.nih.gov

See Assays section.

 

[Ghoul]

Amount of hgh should decrease

One problem is that unlike the studies attached, UGL vials don't have identical starting amounts of rHGH. Filtering prior to analysis removes large aggregates. So if you know with certainty there was precisely 1mg of rHGH in each vial, you can account for this loss and add the "disappeared" rHGH to the measured amount of degradation. But with UGL if one vial is 1.25mg and the other is 1.12mg there's really
no way to determine how much is lost to large aggregates that formed immediately after reconstitution and caught in the filter.

There's also no vacuum sealed control vial to compare these to, unless Jano has one lying around he can use.

Still though, it should be possible to measure greater degradation in the unrefrigerated vial vs the refrigerated one over a week's time.

If you dig into the rHGH oxidation / stability studies, what oxidation really does is make rHGH unfold at much lower temperatures than if it's not oxidized. So the room temp stored vial should degrade faster than the refridgerated one.

 

[Sera]

One problem is that unlike the studies attached, UGL vials don't have identical starting amounts of rHGH. Filtering prior to analysis removes large aggregates. So if you know with certainty there was precisely 1mg of rHGH in each vial, you can account for this loss and add the "disappeared" rHGH to the measured amount of degradation. But with UGL if one vial is 1.25mg and the other is 1.12mg there's really
no way to determine how much is lost to large aggregates that formed immediately after reconstitution and caught in the filter.

There's also no vacuum sealed control vial to compare these to, unless Jano has one lying around he can use.

Still though, it should be possible to measure greater degradation in the unrefrigerated vial vs the refrigerated one over a week's time.

If you dig into the rHGH oxidation / stability studies, what oxidation really does is make rHGH unfold at much lower temperatures than if it's not oxidized. So the room temp stored vial should degrade faster than the refridgerated one.

I suppose I was assuming there would be at least 3 from each to see if there’s anything consistent. Without that it would be tricky to draw any conclusions.

 

[TallandSmall]

Since this "lack of vacuum vial" is an experiment in progress, here's a more practical contribution. A quick protocol to measure what lack of vacuum / oxidation does to rHGH, that is, cause post reconstitution instability that needs to be evaluated over the course of several days post reconstitution. Two studies attached that clearly demonstrate how this happens to rHGH, and why pharma uses an inert gas to prevent lyophilized rHGH from being exposed to oxygen.

In plain english, if your rHGH vial lacks vacuum, the air that's now in the vial (20% oxygen), oxidizes the methionine in the rHGH, making it less durable and degrade (unfold and aggregate) faster once reconstituted than if it was vacuum sealed.

If you reconstitute and immediately measure purity, there probably won't be much of difference noticed.

View attachment 347683View attachment 347684

I filter based on your suggestions and the fact that Jano filters before testing. I have to vent the new vial when filling it up. Is there any difference as far as oxygen exposure? It seems like UGL is getting post-reconstitution oxygen exposure regardless. Or, maybe I should stop filtering so that I can maintain the vacuum in the original vial?

 

[Ateam2023]

didn't @janoshik already kinda debunk this when he said he had vials of hgh at room temperature for 10 years or something crazy like that, but no real degradation took place?

 

[readalot]

Since this "lack of vacuum vial" is an experiment in progress, here's a more practical contribution. A quick protocol to measure what lack of vacuum / oxidation does to rHGH, that is, cause post reconstitution instability that needs to be evaluated over the course of several days post reconstitution. Two studies attached that clearly demonstrate how this happens to rHGH, and why pharma uses an inert gas to prevent lyophilized rHGH from being exposed to oxygen.

In plain english, if your rHGH vial lacks vacuum, the air that's now in the vial (20% oxygen), oxidizes the methionine in the rHGH, making it less durable and degrade (unfold and aggregate) faster once reconstituted than if it was vacuum sealed.

If you reconstitute and immediately measure purity, there probably won't be much of difference noticed.

View attachment 347683View attachment 347684

I'm in. Where are we doing the analysis and how much?

 

[Ateam2023]

I'm in. Where are we doing the analysis and how much?

your DROOLING again

 

[Sampei]

I suppose I was assuming there would be at least 3 from each to see if there’s anything consistent. Without that it would be tricky to draw any conclusions.

4 vials of each

 

[readalot]

your DROOLING again View attachment 347740

Drooling? Nah.

Tired of talking, yeah. "Shit or get off the pot."

There's plenty of bullshit waiting for the pot.

 

[TallandSmall]

didn't @janoshik already kinda debunk this when he said he had vials of hgh at room temperature for 10 years or something crazy like that, but no real degradation took place?

I think we would be looking at the vacuum issue specifically. I personally thinks it’s a QC issue either way, but I’m open to the possibility that it doesn’t affect degradation significantly.

 

[TheLobster]

An unnecessary person has once again come here and interfered with this topic using chatgpt information. Throughout his life, he has never conducted a test or done any professional work on these matters. The only thing he knows is to share links and information from chatgpt.He has no professional knowledge on this subject

I kindly ask you not to share your unnecessary information under this thread anymore hopeless guy

Let us do our testing and share it here. Stop sabotaging

Stop the blah blah already useless man

 

[Sampei]

An unnecessary person has once again come here and interfered with this topic using chatgpt information. Throughout his life, he has never conducted a test or done any professional work on these matters. The only thing he knows is to share links and information from chatgpt.He has no professional knowledge on this subject

I kindly ask you not to share your unnecessary information under this thread anymore hopeless guy

Let us do our testing and share it here. Stop sabotaging

Stop the blah blah already useless man

I'll coordinate with you for the shipment of vials and the payment of Jano.

 

[Sera]

I suppose the only thing that would make this experiment even better would be a control sample with a vacuum.

Although they would have to be from the same batch to be a good control so it might be impossible to obtain.

 

[Spaceman Spiff]

I'll coordinate with you for the shipment of vials and the payment of Jano.

Tired of hypothetical information and leaving room for @Ghoul to be right.

Even pharma can be a off by a small amount of mg.

He wouldn't know that cause he has never tested anything.

Even his precious fucking indian pharma is off on variance testing.