Δ6-testosterone enanthate

[readalot]

its not a impurity. delta6 test e is just slighty more anabolic than androgenic but its still test e.

Testosterone Enantate EP Impurity F | CAS No: NA

Buy high quality Testosterone Enantate EP Impurity F, CAS No: NA from AquigenBio, the leading Pharmaceutical Impurities Manufacturer and Supplier.

aquigenbio.com

I thought it was an impurity. Definitely different steroid. Reference on the anabolic / androgenic features?

 

[readalot]

its not a impurity. delta6 test e is just slighty more androgenic than anabolic but its still test e.

Found similar -ene impurity with Test C. Unclear if HPLC resolves them. Hence the potential project.

R

Post in thread 'Readalots Enhanced Testing'

Nov 8, 2024

Project 3. Forthcoming.

Project Code Name:
An Honest and Ethical Vendor can make a difference.

Why GCMS (along with HPLC) is your friend. Of course some vendors will continue to not test their raws.

Test C and Test E raws are very pure and g2g, right? "Don't look behind the curtain, we'll go back and dial in the concentrations; everything A-ok. Extra stuff on the house!"

View attachment 301207View attachment 301208

Project 3 cont.

Comparison between GCMS purity (area counts approximation) and HPLC purity. Use drop down above to see the previously shared GCMS TIC tables...

• readalot

• 

 

[Sera]

I was wondering if a standard for it is even needed, can’t the usual reference standard for say testosterone just be used to determine if something is different than it… not like we need to clearly identify delta 6 is it? Just want to see what percentage matches exactly with the ‘correct’ reference molecule.

 

[readalot]

I was wondering if a standard for it is even needed, can’t the usual reference standard for say testosterone just be used to determine if something is different than it… not like we need to clearly identify delta 6 is it? Just want to see what percentage matches exactly with the ‘correct’ reference molecule.

We would need to run both reference materials through the HPLC to show if they are resolved. If not, that shows HPLC doesnt give accurate purity results when this impurity is present.

I have no idea the anabolic effect of the -ene impurity.

I don't think it was an artifact of the GCMS testing.

 

[Sera]

We would need to run both reference materials through the HPLC to show if they are resolved. If not, that shows HPLC doesnt give accurate purity results when this impurity is present.

I have no idea the anabolic effect of the -ene impurity.

I don't think it was an artifact of the GCMS testing.

Well, I assume its not resolved or jano wouldn’t have had to do this extended run special test to even discover it right?

 

[dinfar1337]

Testosterone Enantate EP Impurity F | CAS No: NA

Buy high quality Testosterone Enantate EP Impurity F, CAS No: NA from AquigenBio, the leading Pharmaceutical Impurities Manufacturer and Supplier.

aquigenbio.com

I thought it was an impurity. Definitely different steroid. Reference on the anabolic / androgenic features?

caught me with my pants down, i dont know what the fuck it is.

looking it up its just an aas, you can be right in saying its a impurity from testosterone synthesis in the sense its not what you wanted to get but it acts completely as testosterone besides the stuff i will talk about down below.

but it will be metabolized through the liver into testosterone, just as normal test e would be metabolized into testosterone through the p450 enzyme. its basically just testosterone with higher binding affinity to the ar. metabolism rates might slightly differ because its higher binding affinity to the androgen receptor

so all in all, its completely normal testosterone. i dont see how this is the thing causing pip

 

[readalot]

Well, I assume its not resolved or jano wouldn’t have had to do this extended run special test to even discover it right?

We had a long conversation on this. I will try to find it.

 

[readalot]

Well, I assume its not resolved or jano wouldn’t have had to do this extended run special test to even discover it right?

Here you go.

R

Post in thread 'Readalots Enhanced Testing'

Nov 12, 2024

That's a good find.

Appreciate it and my pleasure.

My hypothesis is the Test E reference standard will come through GCMS 99%+ unscathed. I don't think the vast majority of structural impurities detected in the Test ester raws are an artifact of the GCMS measurement. They are present in the raw.

What's your temperature program on the GCMS? 180 to 300 deg C range?

• readalot

• 

Long story short, we need the impurity reference standard to make a definitive conclusion. Jano ran the Test E crm through GCMS and the GC heating couldn't account for amount of -ene impurity found in the raw sample.

I dont think he has the HPLC trace for original data set at the top of this thread. Hence we wanted to do this methodically.

 

[AlexDavis43]

Well, I assume its not resolved or jano wouldn’t have had to do this extended run special test to even discover it right?

HPLC isn't like a switch, yes or no

Conditions can be tweaked to resolve similar compounds and I'm guessing that's why this was a big project at first, but once you establish the conditions, it's business as usual

 

[readalot]

HPLC isn't like a switch, yes or no

Conditions can be tweaked to resolve similar compounds and I'm guessing that's why this was a big project at first, but once you establish the conditions, it's business as usual

This is still an active question mark as far as I am aware. Feel free to go to Janoshik to confirm. Unclear if HPLC method (and column conditions) used today will resolve these two steroids.

 

[dinfar1337]

This is still an active question mark as far as I am aware. Feel free to go to Janoshik to confirm. Unclear if HPLC method (and column conditions) used today will resolve these two steroids.

im not janoshik, but i think the answer is no or he would be our hero already

 

[readalot]

caught me with my pants down, i dont know what the fuck it is.

looking it up its just an aas, you can be right in saying its a impurity from testosterone synthesis in the sense its not what you wanted to get but it acts completely as testosterone besides the stuff i will talk about down below.

but it will be metabolized through the liver into testosterone, just as normal test e would be metabolized into testosterone through the p450 enzyme. its basically just testosterone with higher binding affinity to the ar. metabolism rates might slightly differ because its higher binding affinity to the androgen receptor

so all in all, its completely normal testosterone. i dont see how this is the thing causing pip

Very interesting. So your conclusion is this molecule will be converted to testosterone via cytochrome enzymes.

Yeah, I never made any claims on the PIP. It is just this work by Jano intersected with the stuff we found in the raw on one of the projects. Thanks for the info.

 

[dinfar1337]

Very interesting. So your conclusion is this molecule will be converted to testosterone via cytochrome enzymes.

Yeah, I never made any claims on the PIP. It is just this work by Jano intersected with the stuff we found in the raw on one of the projects. Thanks for the info.

yes, i also looked at janos message and he didnt know what it was(or said he didnt know if it caused pip). it shouldn't do that tho, u can read the description here but its very hard to find anything on this shit since its just byproduct by bad quality testosterone synthesis probably.

https://www.biosynth.com/p/FT28049/2484-30-2-delta6-testosterone

 

[readalot]

but it will be metabolized through the liver into testosterone, just as normal test e would be metabolized into testosterone through the p450 enzyme. its basically just testosterone with higher binding affinity to the ar. metabolism rates might slightly differ because its higher binding affinity to the androgen receptor

This is molecular simulation or how are you making this conclusion?

I thought there were specs on impurity amounts for a reason. This is very useful info you are providing.

 

[readalot]

yes, i also looked at janos message and he didnt know what it was(or said he didnt know if it caused pip). it shouldn't do that tho, u can read the description here but its very hard to find anything on this shit since its just byproduct by bad quality testosterone synthesis probably.

https://www.biosynth.com/p/FT28049/2484-30-2-delta6-testosterone

Wow, thank you for this reference. So according to this there would be no need to include this "impurity" in the impurity spec since it converts to Test once in the body. Very cool. Really appreciate it.

 

[dinfar1337]

Wow, thank you for this reference. So according to this there would be no need to include this "impurity" in the impurity spec since it converts to Test once in the body. Very cool. Really appreciate it.

yes.

looking it up those references they have is completely bullshit leading to urinary detection of oral test u????

but their description is good, you can find limited studies on delta6 test on google scholar describing its metabolism accuring normally in humans.

Google Scholar

scholar.google.com

theres not that many studies on it, since its just gonna end up as testosterone anyways, just more aggresively binding to the ar.

 

[readalot]

yes.

looking it up those references they have is completely bullshit leading to urinary detection of oral test u????

but their description is good, you can find limited studies on delta6 test on google scholar describing its metabolism accuring normally in humans.

Google Scholar

scholar.google.com

theres not that many studies on it, since its just gonna end up as testosterone anyways, just more aggresively binding to the ar.

I totally neglected the downstream fate of this molecule. Very much appreciated Sir. Thank you very much!

Saved us a bundle of money.

 

[dinfar1337]

I totally neglected the downstream fate of this molecule. Very much appreciated Sir. Thank you very much!

Saved us a bundle of money.

haha i simply did nothing, i was interested in the topic when i caught wind of this thread and looked it up. but it just seemed to be a fluke.

my guess would be either; its way more complicated or there isnt a problem(i have never gotten pip with test e so dont get mad at me now) with the raws.

if you find anything that could be credible i wouldn't mind looking into it and if it looks credible enough i will gladly toss some bitcoin over to get it tested

 

[Synotic]

I see....

This is incredible stuff. My next question would be, does this delta 6 variant metabolizes any differently in the body, or exactly the same as its counterpart?

Thank you for this discovery.

I am always learning something new here on Meso.

I asked myself the same question. Here‘s what chatgpt had to say, however, i‘d just take this info with a grain of salt:

Summary:


Delta-6 testosterone is basically a structural isomer of testosterone where the double bond is at C6 instead of C4.

• Less aromatization → much less estrogenic activity.
• Doesn’t reduce efficiently to DHT.
• Weaker androgenic potency than testosterone, but retains anabolic activity.
• Considered a “milder, drier” anabolic compared to natural testosterone.

 

[xrayphoton13]

I asked myself the same question. Here‘s what chatgpt had to say, however, i‘d just take this info with a grain of salt:

Summary:


Delta-6 testosterone is basically a structural isomer of testosterone where the double bond is at C6 instead of C4.

• Less aromatization → much less estrogenic activity.
• Doesn’t reduce efficiently to DHT.
• Weaker androgenic potency than testosterone, but retains anabolic activity.
• Considered a “milder, drier” anabolic compared to natural testosterone.

I gotta call bullshit on this entire post until proven otherwise. Your screenshots keep saying studies show this and that. Delta 6 testosterone was only a recently discovered degradation product from newer batches of test e. There's just no way pure raws of this have been synthesized let alone studied in humans.