MESO-Rx
Anabolic Steroids
There is any way to sterilize vials without and autoclave?
- Thread starter abraao25
- Start date
An autoclave is basically a pressure cooker and the latter can be used for sterilization.
There's also just dry heat, i.e an oven, it just takes a lot longer.
There's also just dry heat, i.e an oven, it just takes a lot longer.
Mike Oxbig
New Member
This.
An autoclave is entirely unnecessary unless you're planning on distributing. A lot of UGLs don't even have an autoclave.
Stretch
Subscriber
The answer to the question is NO probably!
OK so products be sterilized absent an autoclave sure they can be! How thru the use of chemicals primarily. As Stretch mentioned few UGL, scratch that, NO UGL uses strict aseptic technique. Why because they don't need to!
Briefly there are three factors which will determine whether someone acquires an infection after pinning.
1) the number of bacteria injected
2) the virulence of those bacteria
3) the immune state of the host
Ok we all know the water we drink is not sterile BUT even in this circumstance the number of bacteria DOES matter. Consequently drinking water is tested for bacterial counts AND any TOXIC bacteria.
Now the same is obviously true of injected medications yet those factors I mentioned above are even more critical because most of the protective functions of the immune system has been bypassed.
ERGO, UGLs use "filtering paper" and pressure seives much like campers use to
clean brackish water. Obviously the cleaner the SOLVENT (liquid) and SOLUTE (AAS) the less complicated the entire process becomes.
Back to your question. The primary problem in medicine historically was the destruction of bacterial SPORES and viruses. This is accomplished with EITHER an AUTOCLAVE or chemical reagents (Hebiclens, Alcohol, Betadine or gases such as ethelene oxide).
Of course one of the major problems with these chemical is they may also be TISSUE TOXIC and cause damage themselves. So now you will need a sterile cleaning product such as water, a sterile basin, sterile towels, sterile etc.
The problem for the novice or UGL? To maintain absolute sterility EVERYTHING which comes in contact with that STERILE item must also be sterile. Now we have a relatively complicated and expensive.
So the AAS from UGLs are not sterile BUT IF they do their job correctly and we are knowledgeable about how to perform a injection properly infections are quite unusual and the MAJORITY (prob 90% IME) is the result of MIS-INFORMED,, HURRIED, or CARLESS TECHNIQUE.
JIM
Briefly there are three factors which will determine whether someone acquires an infection after pinning.
1) the number of bacteria injected
2) the virulence of those bacteria
3) the immune state of the host
Ok we all know the water we drink is not sterile BUT even in this circumstance the number of bacteria DOES matter. Consequently drinking water is tested for bacterial counts AND any TOXIC bacteria.
Now the same is obviously true of injected medications yet those factors I mentioned above are even more critical because most of the protective functions of the immune system has been bypassed.
ERGO, UGLs use "filtering paper" and pressure seives much like campers use to
clean brackish water. Obviously the cleaner the SOLVENT (liquid) and SOLUTE (AAS) the less complicated the entire process becomes.
Back to your question. The primary problem in medicine historically was the destruction of bacterial SPORES and viruses. This is accomplished with EITHER an AUTOCLAVE or chemical reagents (Hebiclens, Alcohol, Betadine or gases such as ethelene oxide).
Of course one of the major problems with these chemical is they may also be TISSUE TOXIC and cause damage themselves. So now you will need a sterile cleaning product such as water, a sterile basin, sterile towels, sterile etc.
The problem for the novice or UGL? To maintain absolute sterility EVERYTHING which comes in contact with that STERILE item must also be sterile. Now we have a relatively complicated and expensive.
So the AAS from UGLs are not sterile BUT IF they do their job correctly and we are knowledgeable about how to perform a injection properly infections are quite unusual and the MAJORITY (prob 90% IME) is the result of MIS-INFORMED,, HURRIED, or CARLESS TECHNIQUE.
JIM
Stayin Small
New Member
What's the deal with UV radiation? On a larger job I did (electrician), I installed and wired up some pretty expensive UV light fixtures, for what appeared to be sterilization purposes. I'm not a chemist, or a microbiologist, I've just never seen anybody on here mention this method.
loveyourbody
New Member
Dr Jim that was a very good post lots of information. I would have to agree with the host immune system when I first started using gear I did a lot of stupid things but I was lucky and never got an infection or pip. I didn't disinfect the top of the vial or injection site. Which now I would never inject without cleaning the site. But, one of my close friends did a cycle about 7months ago and did the same thing. He got an infection.
Life lessons
Life lessons
whwlives
New Member
6 hours (no less) in an oven at 270F minimum will sterilize vials.
Septa (and everything not glass) will need an autoclave, and a "pressure cooker" IS NOT AN AUTOCLAVE.
A decent stove-top autoclave is under $400 on Amazon.
whw
Septa (and everything not glass) will need an autoclave, and a "pressure cooker" IS NOT AN AUTOCLAVE.
A decent stove-top autoclave is under $400 on Amazon.
whw
fatbastard1
New Member
I did beer bottles with a common bleach based antibacterial cleaner found in all homebrew shops then washed with clean water.Id use this attempt.
Yes, but you don't inject beer. The point of sterilizing the bottles is to kill beasts & yeasts that might spoil the brew. The alcohol in the beer will take care of most bacteria, and your gut can fight off the rest. An injectable bypasses the skin and the gut.
Heat + time, heat + pressure + time, or heat + pressure + time + chemicals.
Heat + time, heat + pressure + time, or heat + pressure + time + chemicals.
Beer? What are you talking about? Beer contains both high bacteria and yeasts counts, making it anything but sterile!
What form of ethyl alcohol then?
EVERCLEAR which is about 95% ETOH.
At such high concentrations probably the only survivors will be bacterial spores.
I would suggest rinsing the vials with sterile water OR saline after the Everclear bath since any alcohol at a 95% concentration is tissue toxic AND will add to PIP.
What form of ethyl alcohol then?
EVERCLEAR which is about 95% ETOH.
At such high concentrations probably the only survivors will be bacterial spores.
I would suggest rinsing the vials with sterile water OR saline after the Everclear bath since any alcohol at a 95% concentration is tissue toxic AND will add to PIP.
mr.nitro
New Member
With common household items, your not going to get a true sterile product. I mean even touching the bottle contaminates the sterile field. Typical Rubber gloves are not sterile either. Special sterile gloves are individually wrapped and must be put on extremely carefully, basically slipping into them without touching the outside.
That being said, no home brew can claim to be a true sterile product. Thus why some alcohol must be added to the batch.
What I have used in the past was an alcohol bath and allowed to air dry. I've used my own brew for many years without ever getting an infection. My ba to bb ratio was 2% ba and 20% bb
That being said, no home brew can claim to be a true sterile product. Thus why some alcohol must be added to the batch.
What I have used in the past was an alcohol bath and allowed to air dry. I've used my own brew for many years without ever getting an infection. My ba to bb ratio was 2% ba and 20% bb
According to [ame="http://en.wikipedia.org/wiki/Ultraviolet_germicidal_irradiation"]Wikipedia[/ame], if you're disinfecting liquids, you have to filter it pretty well to remove particles that could contain bacteria. It also points out that this is a disinfecting (not "sterilizing") process, and is often used in conjunction with other methods.
So I'd guess that it doesn't really add anything to the process if you're going to ultrafilter the oil anyway. I'd also wonder if the UV could damage the steroid.
It's not only home brewed products that contain some alcohol, pharmaceutical products contain it as well.
Although Pfizer and Bayer don't use this much.
mr.nitro
New Member
I don't doubt they use different ratio's of solvents. That's just what I prefer to use.
I personally alchoal bath place upside down in oven on a try with baking sheet, heat for 30 min or so pull out rinse with distilled bottle water repeat process atleast twice. Rinse with distilled water again and place back in oven upside down on the baking sheet till dry.
filtration and an oven bro.
swole4ever
New Member
How about sterilizing the butyl caps?
Dry sterilization 170 degrees C for 1 hour.
VALIDATION OF DRY HEAT STERILIZATION METHODS | PharmaTutor
VALIDATION OF DRY HEAT STERILIZATION METHODS | PharmaTutor
