JoeAverage
New Member
Hi all,
I’ve been following some of the GLP-1 discussions for a while and had a technical question regarding Reta quality control.
For compounds like semaglutide or tirzepatide, most people seem reasonably comfortable with a standard RP-HPLC purity report (often combined with third-party testing).
Reta, however, is a longer and more structurally complex peptide.
My understanding is that typical RP-HPLC primarily shows the purity profile and retention time consistency, but it doesn’t by itself confirm full sequence accuracy or site-specific modifications — which, in some cases, could affect biological activity.
HPLC is clearly a necessary baseline. I’m just wondering whether it’s sufficient on its own for a peptide of this length and complexity, or if LC-MS confirmation or peptide mapping would generally be expected for higher confidence.
Not suggesting there’s an issue — just trying to better understand what level of analytical scrutiny makes sense for longer-chain peptides like this.
Curious to hear thoughts from those with more experience on the analytical side.
I’ve been following some of the GLP-1 discussions for a while and had a technical question regarding Reta quality control.
For compounds like semaglutide or tirzepatide, most people seem reasonably comfortable with a standard RP-HPLC purity report (often combined with third-party testing).
Reta, however, is a longer and more structurally complex peptide.
My understanding is that typical RP-HPLC primarily shows the purity profile and retention time consistency, but it doesn’t by itself confirm full sequence accuracy or site-specific modifications — which, in some cases, could affect biological activity.
HPLC is clearly a necessary baseline. I’m just wondering whether it’s sufficient on its own for a peptide of this length and complexity, or if LC-MS confirmation or peptide mapping would generally be expected for higher confidence.
Not suggesting there’s an issue — just trying to better understand what level of analytical scrutiny makes sense for longer-chain peptides like this.
Curious to hear thoughts from those with more experience on the analytical side.
