Bottle top filter method contaminated ??

[findusparker]

Hello guys...

This is how I see it, please correct a man if he is wrong... you finish mixing and warming your test/tren brew, adding your 1%BA/20%BB (or whatever it is). Its time to suck it through your Minipore or Nalgene or whatever bottle top you use. It comes through, just like it should into the receiver bottle.

Heres my problem....

You have to take the filter kit off the receiver bottle, contaminated air enters the filtered gear. Stick in your 50ml syringe with the 20g tip and squirt into sterile vials with a breather tip sticken out of them. Pluis, the receiver flask is gunna be open to the elements for the couple of minutes it takes to draw and squirt your gear into the vials if you have a few.

Am I missing something ? Is there a step Ive skipped ? My understanding of the BA is to give an increased shelf life, whilst BB keeps the gear nice and dissolved. Sooo....

did we just waste our time filtering only to recontaminate by opening the bottle ??

 

[Kamala]

Hello guys...

This is how I see it, please correct a man if he is wrong... you finish mixing and warming your test/tren brew, adding your 1%BA/20%BB (or whatever it is). Its time to suck it through your Minipore or Nalgene or whatever bottle top you use. It comes through, just like it should into the receiver bottle.

Heres my problem....

You have to take the filter kit off the receiver bottle, contaminated air enters the filtered gear. Stick in your 50ml syringe with the 20g tip and squirt into sterile vials with a breather tip sticken out of them. Pluis, the receiver flask is gunna be open to the elements for the couple of minutes it takes to draw and squirt your gear into the vials if you have a few.

Am I missing something ? Is there a step Ive skipped ? My understanding of the BA is to give an increased shelf life, whilst BB keeps the gear nice and dissolved. Sooo....

did we just waste our time filtering only to recontaminate by opening the bottle ??

In theory yes...... Although its kind of the same thing when you open an amp, the whole thing is exposed to the open air till you get it drawn into the syringe. I've probably used a hundred amps and never had an issue.

If your doing it on a small scale just use a big sterile vial with a stopper already in it, and a whatman attached..... There's zero exposure to air this way.... Its going from a sterile filter directly into a sterile vial.

 

[Dr JIM]

About the only time laminar flow is used in medicine is when Parenteral drugs are being reconstituted for severely immune compromise patients, since the number of atmospheric bacteria is MINUSCULE.

Ergo should them critters enter the vial and some no doubt do, immune competent patients have absolutely NO DIFFICULTY wiping them bastards out.

Moreover regardless of how hard you swab or scrub your dermis with ETOH, Hebiclens and or Dial soap more bacteria will enter your body as a result of pinning than atmospheric bugs entering that vial.

What is the leading bug on post operative infections? Staph Aureus! What is it's origin? The dermis!

Heck u think injection abscesses come from that vial NOT, it's almost always due to sloppy "sterile" injection technique!

 

[findusparker]

In theory yes...... Although its kind of the same thing when you open an amp, the whole thing is exposed to the open air till you get it drawn into the syringe. I've probably used a hundred amps and never had an issue.

If your doing it on a small scale just use a big sterile vial with a stopper already in it, and a whatman attached..... There's zero exposure to air this way.... Its going from a sterile filter directly into a sterile vial.

Thanks so much for the reply guys, I so appreciate it. When you open an amp, its only exposed for a minute, then its pinned. In a minute i would think bacteria would have enough time to grow/manifest.

The difference is (I think) after you expose the filtered gear from the receiver bottle, and then transfer it into the sterile vial, the air it was exposed to apon opening the reciever botthe, has much longer to grow within the sterile vial. I could be way off.... If im retarted let me know.

I got all the equipment for the bottle top filter assembly. Next time ill take ya advise and go for the syringe filter method.

 

[findusparker]

About the only time laminar flow is used in medicine is when Parenteral drugs are being reconstituted for severely immune compromise patients, since the number of atmospheric bacteria is MINUSCULE.

Ergo should them critters enter the vial and some no doubt do, immune competent patients have absolutely NO DIFFICULTY wiping them bastards out.

Moreover regardless of how hard you swab or scrub your dermis with ETOH, Hebiclens and or Dial soap more bacteria will enter your body as a result of pinning than atmospheric bugs entering that vial.

What is the leading bug on post operative infections? Staph Aureus! What is it's origin? The dermis!

Heck u think injection abscesses come from that vial NOT, it's almost always due to sloppy "sterile" injection technique!

Never have I felt like such a rookie haha. What is Laminar flow, parental drugs, ergo, dermis, ETOH, Aureus.... I have no idea but I think what your trying to tell me is that the lid off the receiver bottle whilst im drawing product into my 50ml syringes is not significant enough for airborn bacteria to spoil my gear after I put it into vials. To dumb it down further...Im worrying about nothing.

And to furthermore demonstrate your point, your pointing out that the source of pinning infections is mostly due to poor hygienic pinning, rather than limited bacteria in the gear... so little in fact that the immune system should kill it easy enough ?

That interpreted about right ?

 

[Kamala]

Thanks so much for the reply guys, I so appreciate it. When you open an amp, its only exposed for a minute, then its pinned. In a minute i would think bacteria would have enough time to grow/manifest.

The difference is (I think) after you expose the filtered gear from the receiver bottle, and then transfer it into the sterile vial, the air it was exposed to apon opening the reciever botthe, has much longer to grow within the sterile vial. I could be way off.... If im retarted let me know.

I got all the equipment for the bottle top filter assembly. Next time ill take ya advise and go for the syringe filter method.

I'm no expert on this by any means lol....... If Jim says your GTG, I'd take him at his word.

 

[Dr JIM]

Laminar flow = in this instance is controlled filtered air movement which greatly decreases bacterial "settlement".

Ergo = therefore
ETOH = alcohol
Staph Aureus = the most abundant bacteria found on human skin
Dermis. = skin
Parenteral drugs = those drugs administered by the , intramuscular, intravenous, subcutaneous or intrathecal (into spinal fluid) route





Now that you have learned something I'll answer your question.








Air borne bacteria will pose NO PROBLEM!!!!!!

 

[findusparker]

Thanks so much seriously guys. This has been bugging me and I knew I'd look like an amature but just needed to feel better about it.

Thanks again

 

[Dr JIM]

Oh yea that answer applies only if you are NOT, an diabetic AIDS patient with cancer and tuberculosis, because then, "Houston we have a problem" is a fitting colloquialism (saying).

 

[findusparker]

What's a colloquialism ? Kidding. Actually Ill look it up

 

[Mr. Joe]

In research we use Laminar Flow hood's all of the time for tissue culture, and filter media ( .22um in the hood) because cells have no immune system. I agree with Jim, you're probably okay as long as you're not doing this sick or sneezing in the bottle etc. But for piece of mind I would keep it sterile from start to finish as in use a syringe filter into a sterile vial.

 

[tns]

Now that ive used a syringe filter myself, im telling you, it needs some patience.
bottle top sounds a lot easier